Identification of circRNAs for miRNA targets by argonaute2 RNA immunoprecipitation and luciferase screening assays / Yan Li, Bing Chen, and Shenglin Huang. Deep computational circular RNA analytics from RNA-seq data / Tobias Jakobi and Christoph Dieterich -- Genome-wide circRNA profiling from RNA-seq data / Daphne A. Cooper, Mariela Cortés-López, and Pedro Miura -- Analysis of circular RNAs using the web tool CircInteractome / Amaresh C. Panda, Dawood B. Dudekula, Kotb Abdelmohsen, and Myriam Gorospe -- Characterization and validation of circular RNA and their host gene mRNA expression using PCR / Andreas W. Heumüller and Jes-Niels Boeckel -- Detecting circular RNAs by RNA fluorescence in situ hybridization / Anne Zirkel and Argyris Papantonis -- Single-molecule fluorescence in situ hybridization (FISH) of circular RNA CDR1as / Christine Kocks, Anastasiya Boltengagen, Monika Piwecka, Agnieszka Rybak-Wolf, and Nikolaus Rajewsky -- Highly efficient strategy for overexpressing circRNAs / Dawei Liu, Vanessa Conn, Gregory J. Goodall, and Simon J. Conn -- Constructing GFP-based reporter to study back splicing and translation of circular RNA / Yun Yang and Zefeng Wang -- Northern blot analysis of circular RNAs / Tim Schneider, Silke Schreiner, Christian Preusser, Albrecht Bindereif, and Oliver Rossbach -- Nonradioactive northern blot of circRNAs / Xiaolin Wang and Ge Shan -- Characterization of circular RNA concatemers / Thomas B. Hansen -- Characterization of circular RNAs (circRNA) associated with the translation machinery / Deniz Bartsch, Anne Zirkel, and Leo Kurian -- Synthesis and engineering of circular RNAs / Sonja Petkovic and Sabine Müller -- Preparation of circular RNA in vitro / Naoko Abe, Ayumi Kodama, and Hiroshi Abe -- Discovering circRNA-microRNA interactions from CLIP-seq data / Xiao-Qin Zhang and Jian-Hua Yang -- Identification of circRNAs for miRNA targets by argonaute2 RNA immunoprecipitation and luciferase screening assays / Yan Li, Bing Chen, and Shenglin Huang.
Summary:
This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico.èapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circular RNAs: Methods and Protocols aims to useful and informative for further study into this vital field.
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.