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04259aam a2200385 i 4500 001 ABBE2380580511E8A8F83C5097128E48 003 SILO 005 20180515010114 008 171004t20182018nyua b 001 0deng d 010 $a 2017962947 020 $a 1493975617 020 $a 9781493975617 035 $a (OCoLC)1005103525 040 $a YDX $b eng $e rda $c YDX $d OCLCO $d GZM $d NUI $d SILO 245 00 $a Circular RNAs : $b methods and protocols / $c edited by Christoph Dieterich, Argyris Papantonis. 264 1 $a New York, NY : $b Humana Press, $c [2018] 300 $a xiii, 220 pages : $b illustrations (some color) ; $c 26 cm. 490 1 $a Springer protocols, $x 1949-2448 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v volume 1724 504 $a Includes bibliographical references and index. 505 00 $t Identification of circRNAs for miRNA targets by argonaute2 RNA immunoprecipitation and luciferase screening assays / $r Yan Li, Bing Chen, and Shenglin Huang. $t Deep computational circular RNA analytics from RNA-seq data / $r Tobias Jakobi and Christoph Dieterich -- $t Genome-wide circRNA profiling from RNA-seq data / $r Daphne A. Cooper, Mariela CorteÌs-LoÌpez, and Pedro Miura -- $t Analysis of circular RNAs using the web tool CircInteractome / $r Amaresh C. Panda, Dawood B. Dudekula, Kotb Abdelmohsen, and Myriam Gorospe -- $t Characterization and validation of circular RNA and their host gene mRNA expression using PCR / $r Andreas W. HeumuÌller and Jes-Niels Boeckel -- $t Detecting circular RNAs by RNA fluorescence in situ hybridization / $r Anne Zirkel and Argyris Papantonis -- $t Single-molecule fluorescence in situ hybridization (FISH) of circular RNA CDR1as / $r Christine Kocks, Anastasiya Boltengagen, Monika Piwecka, Agnieszka Rybak-Wolf, and Nikolaus Rajewsky -- $t Highly efficient strategy for overexpressing circRNAs / $r Dawei Liu, Vanessa Conn, Gregory J. Goodall, and Simon J. Conn -- $t Constructing GFP-based reporter to study back splicing and translation of circular RNA / $r Yun Yang and Zefeng Wang -- $t Northern blot analysis of circular RNAs / $r Tim Schneider, Silke Schreiner, Christian Preusser, Albrecht Bindereif, and Oliver Rossbach -- $t Nonradioactive northern blot of circRNAs / $r Xiaolin Wang and Ge Shan -- $t Characterization of circular RNA concatemers / $r Thomas B. Hansen -- $t Characterization of circular RNAs (circRNA) associated with the translation machinery / $r Deniz Bartsch, Anne Zirkel, and Leo Kurian -- $t Synthesis and engineering of circular RNAs / $r Sonja Petkovic and Sabine MuÌller -- $t Preparation of circular RNA in vitro / $r Naoko Abe, Ayumi Kodama, and Hiroshi Abe -- $t Discovering circRNA-microRNA interactions from CLIP-seq data / $r Xiao-Qin Zhang and Jian-Hua Yang -- $t Identification of circRNAs for miRNA targets by argonaute2 RNA immunoprecipitation and luciferase screening assays / $r Yan Li, Bing Chen, and Shenglin Huang. 520 $a This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico.eÌapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circular RNAs: Methods and Protocols aims to useful and informative for further study into this vital field. 650 0 $a RNA $v Laboratory manuals. 650 2 $a RNA. $0 (DNLM)D012313 655 7 $a Laboratory manuals. $2 lcgft 655 2 $a Laboratory Manuals. $0 (DNLM)D020484 700 1 $a Dieterich, Christoph, $e editor. 700 1 $a Papantonis, Argyris, $e editor. 776 08 $i Electronic version: $t Circular RNAs. $d New York, NY : Humana Press ; Springer, [2018] $z 9781493975624 $w (OCoLC)1019158735 773 18 $w 990001282000202771 $g v:1724 830 0 $a Springer protocols (Series), $x 1949-2448 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1724. $x 1064-3745 941 $a 1 952 $l OVUX522 $d 20181116030515.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=ABBE2380580511E8A8F83C5097128E48Initiate Another SILO Locator Search