Production, purification, and titration of first-generation adenovirus vectors / Ramona F. Kratzer and Florian Kreppel. RNA function prediction / Yongsheng Li, Juan Xu, Tingting Shao, Yunpeng Zhang, Hong Chen, and Xia Li -- Computational prediction of novel miRNAs from genome-wide data / Georgina Stegmayer, Cristian Yones, Laura Kamenetzky, Natalia Macchiaroli, and Diego H. Milone -- Protein structure modeling with MODELLER / Benjamin Webb and Andrej Sali -- Protein function prediction / Leonardo Magalhaes Cruz, Sheyla Trefflich, Vinicius Almir Weiss, and Mauro Antonio Alves Castro -- Capturing three-dimensional genome organization in individual cells by single-cell hi-c / Takashi Nagano, Steven W. Wingett, and Peter Fraser -- Genome-wide cell type-specific mapping of in vivo chromatin protein binding using an FLP-inducible DamID system in Drosophila / Alexey V. Pindyurin -- DNA methylation profiling using long-read single molecule real-time bisulfite sequencing (SMRT-BS) / Yao Yang and Stuart A. Scott -- Copy number variation analysis by droplet digital PCR / Suvi K. H€arm€al€a, Robert Butcher, and Chrissy H. Roberts -- MicroScale thermophoresis : a rapid and precise method to quantify protein-nucleic acid interactions in solution / Adrian Michael Mueller, Dennis Breitsprecher, Stefan Duhr, Philipp Baaske, Thomas Schubert, and Gernot L€angst -- Establishment of the CRISPR/Cas9 system for targeted gene disruption and gene tagging / Eric Ehrke-Schulz, Maren Schiwon, Claudia Hagedorn, and Anja Ehrhardt -- Holistic and affordable analyses of MicroRNA expression profiles using tagged cDNA libraries and a multiplex sequencing strategy / Patrick P. Weil, Yan Jaszczyszyn, Anne Baroin-Tourancheau, Jan Postberg, and Laurence Amar -- MicroRNA expression analysis using small RNA sequencing discovery and RT-qPCR-based validation / Alan Van Goethem, Pieter Mestdagh, Tom Van Maerken, and Jo Vandesompele -- Using FirePlex particle technology for multiplex microRNA profiling without RNA purification / Michael R. Tackett and Izzuddin Diwan -- Multiplex real-time PCR using encoded microparticles for microRNA profiling / Seungwon Jung and Sang Kyung Kim -- Optimized whole transcriptome profiling of motor axons / Lena Saal-Bauernschubert, Michael Briese, and Michael Sendtner -- 2D-DIGE in proteomics / Matias Pasquali, Tommaso Serchi, Sebastien Planchon, and Jenny Renaut -- STAGE-diging in proteomics / Paolo Soffientini and Angela Bachi -- Protein arrays I : antibody arrays / Yulin Yuan, Zuan-Tao Lin, Hongting Wang, Xia Hong, Mikala Heon, and Tianfu Wu -- Protein arrays II : antigen arrays / Yulin Yuan, Hongting Wang, Zuan-Tao Lin, Xia Hong, Mikala Heon, and Tianfu Wu -- Protein arrays III : reverse-phase protein arrays / Yulin Yuan, Xia Hong, Zuan-Tao Lin, Hongting Wang, Mikala Heon, and Tianfu Wu -- Isolation of exosomes for the purpose of protein cargo analysis with the use of mass spectrometry / Monika Pietrowska, Sonja Funk, Marta Gawin, Łukasz Marczak, Agata Abramowicz, Piotr Widłak, and Theresa Whiteside -- Virus-induced gene silencing (VIGS) and foreign gene expression in Pisum sativum L. Using the "One-Step" Bean pod mottle virus (BPMV) viral vector / Chouaıb Meziadi, Sophie Blanchet, Valerie Geffroy, and Stephanie Pflieger -- Re-expressing epigenetically silenced genes by inducing DNA demethylation through targeting of ten-eleven translocation 2 to any given genomic locus / Julio Cesar Rendon, David Cano-Rodrıguez, and Marianne G. Rots -- Knockdown of rice microRNA166 by short tandem target mimic (STTM) / Sachin Teotia, Dabing Zhang, and Guiliang Tang -- RNAi-mediated knockdown of protein expression / Volker Baumann, Cornelia Lorenzer, Michael Thell, Anna-Maria Winkler, and Johannes Winkler -- Engineered zinc finger DNA-binding domains : synthesis, assessment of DNA-binding affinity, and direct protein delivery to mammalian cells / Mir A. Hossain, Isaac J. Knudson, Shaleen Thakur, Yong Shen, Jared R. Stees, Joeva J. Barrow, and Jorg Bungert -- Production, purification, and titration of first-generation adenovirus vectors / Ramona F. Kratzer and Florian Kreppel.
Series:
Methods in molecular biology, 1064-3745 ; 1654 Springer protocols, 1949-2478
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.