Identification of deacetylase substrates with the biotin switch approach / Matthew J. Rardin, Jason M. Held, and Bradford W. Gibson -- Sirtuins in yeast : phenotypes and tools / Scott Tsuchiyama [and four others] -- C. elegans sirtuins / Mohan Viswanathan and Heidi A. Tissenbaum -- Genetic and biochemical tools for investigating sirtuin function in Drosophila melanogaster / Jason G. Wood, Rachel Whitaker, and Stephen L. Helfand -- Generating mammalian sirtuin tools for protein-interaction analysis / Kathleen A. Hershberger [and three others] -- Mass spectrometry-based detection of protein acetylation / Yu Li [and three others] -- SILAC-based quantification of Sirt1-responsive lysine acetylome / Yue Chen, Gozde Colak, and Yingming Zhao -- Targeted quantitation of acetylated lysine peptides by selected reaction monitoring mass spectrometry / Matthew J. Rardin, Jason M. Held, and Bradford W. Gibson -- Identification of deacetylase substrates with the biotin switch approach / Dongning Wang [and three others] -- The emerging links between sirtuins and autophagy / Lei Zhong [and four others] -- Measurement of sirtuin enzyme activity using a substrate-agnostic fluorometric nicotinamide assay / Basil P. Hubbard and David A. Sinclair -- Detecting sirtuin-catalyzed deacylation reactions using 32P-labeled NAD and thin-layer chromatography / Anita Zhu, Xiaoyang Su, and Hening Lin -- Chemical acetylation and deacetylation / Kristofer S. Fritz -- Accurate measurement of nicotinamide adenine dinucleotide (NAD+) with high-performance liquid chromatography / Jun Yoshino and Shin-ichiro Imai -- In vivo measurement of the acetylation state of sirtuin substrates as a proxy for sirtuin activity / John Dominy, Pere Puigserver, and Carles Cantó -- Oxygen flux analysis to understand the biological function of sirtuins / Dongning Wang [and three others] -- The emerging links between sirtuins and autophagy / Jessica Curtis and Rafael de Cabo. Utilizing calorie restriction to evaluate the role of sirtuins in healthspan and lifespan of mice / Paloma Martínez-Redondo and Alejandro Vaquero -- Circadian measurements of sirtuin biology / Kathryn Moynihan Ramsey [and five others] -- Utilizing calorie restriction to evaluate the role of sirtuins in healthspan and lifespan of mice / Jessica Curtis and Rafael de Cabo.
Series:
Methods in molecular biology, 1064-3745 ; 1077 Springer protocols, 1949-2448
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