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Author:
Sambrook, Joseph.
Title:
Molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell.
Edition:
3rd ed.
Publisher:
Cold Spring Harbor Laboratory Press,
Copyright Date:
c2001
Description:
3 v. : ill. ; 28 cm.
Subject:
Molecular cloning--Laboratory manuals.
Clonage moleĢculaire--Manuels de laboratoire.
Cloning, Molecular--Laboratory Manuals.
Clonamiento molecular--Manuales de laboratorio.
Kloneren.
Genetische manipulatie.
Other Authors:
Russell, David W. (David William), 1957-
Notes:
Includes bibliographical references and index.
Contents:
Vol. 1: Ch. 1. Plasmids and their usefulness in molecular cloning -- Ch. 2. Bacteriophage and its vectors -- Ch. 3. Working with bacteriophage M13 vectors -- Ch. 4. Working with high-capacity vectors -- Ch. 5. Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Ch. 6. Preparation and analysis of eukaryotic genomic DNA -- Ch. 7. Extraction, purification, and analysis of mRNA from eukaryotic cells -- Vol. 2: Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction -- Ch. 9. Preparation of radiolabeled DNA and RNA probes -- Ch. 10. Working with synthetic oligonucleotide probes -- Ch. 11. Preparation of cDNA libraries and gene identification -- Ch. 12. DNA sequencing -- Ch. 13. Mutagenesis -- Ch. 14. Screening expression libraries -- Vol. 3: Ch. 15. Expression of cloned genes in Escherichia coli -- Ch. 16. Introducing cloned genes into cultured mammalian cells -- Ch. 17. Analysis of gene expression in cultured mammalian cells -- Ch. 18. Protein interaction technologies.
ISBN:
9780879695767 (cloth)
0879695765 (cloth)
9780879695774 (paper)
0879695773 (paper)
OCLC:
(OCoLC)45015638
LCCN:
00064380
Locations:
USUX851 -- Iowa State University - Parks Library (Ames)
UDAX314 -- Charles C. Myers Library (Dubuque)
OVUX522 -- University of Iowa Libraries (Iowa City)
O3AX572 -- Cornell College - Russell D. Cole Library (Mount Vernon)
OUAX845 -- Dordt University (Sioux Center)

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