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Title:

Synthetic messenger RNA and cell metabolism modulation : methods and protocols / edited by Peter M. Rabinovich.

Publisher:

Humana Press ;

Copyright Date:

c2013

Description:

xi, 323 p. : ill. (some col.) ; 27 cm.

Subject:

RNA, Messenger.
Cell Engineering.
Cell Physiological Processes.
Cells--metabolism.
Gene Transfer Techniques.
Messenger RNA.
Cell metabolism.

Other Authors:

Rabinovich, Peter M.

Notes:

Includes bibliographical references and index.

Contents:

Cell engineering with synthetic messenger RNA / Peter M. Rabinovich and Sherman M. Weissman -- In vitro transcription of long RNA containing modified nucleosides / Norbert Pardi ... [et al.] -- HPLC purification of in vitro transcribed long RNA / Drew Weissman ... [et al.] -- Synthetic mRNAs with superior translation and stability properties / Ewa Grudzien-Nogalska ... [et al.] -- Nonviral, cationic lipid-mediated delivery of mRNA / James G. Hecker -- Light-induced mRNA transfection / Sigurd Leinæs Bøe and Eivind Hovig -- Engineering B cells with mRNA / Jaewoo Lee, David Boczkowski, and Smita Nair -- Programming human dendritic cells with mRNA / Jaewoo Lee, David Boczkowski, and Smita Nair -- Large volume flow electroporation of mRNA : clinical scale process / Linhong Li ... [et al.] -- Retrovirus-based mRNA transfer for transient cell manipulation / Melanie Galla, Axel Schambach, and Christopher Baum --
mRNA delivery to human dendritic cells by recombinant yeast and activation of antigen-specific memory T cells / Frank Breinig, Tanja Breinig, and Manfred J. Schmitt -- Nonviral RNA transfection to transiently modify T cells with chimeric antigen receptors for adoptive therapy / Tobias Riet ... [et al.] -- Natural killer cell reprogramming with chimeric immune receptors / Noriko Shimasaki and Dario Campana -- Reprogramming to pluripotency and differentiation of cells with synthetic mRNA / Peizhe Wang and Jie Na -- Antitumor vaccination with synthetic mRNA : strategies for in vitro and in vivo preclinical studies / Mustafa Diken ... [et al.] -- Mannosylated and histidylated LPR technology for vaccination with tumor antigen mRNA / Chantal Pichon and Patrick Midoux -- Analysis of survivin-specific T cells in breast cancer patients using human DCs engineered with survivin mRNA / Ozcan Met and Inge Marie Svane --
mRNA electroporation as a tool for immunomonitoring / Nathalie Cools ... [et al.] -- mRNA PCR-based epitope chase method / Jean-Daniel Doucet, Dominique Gauchat, and Rejean Lapointe.

Series:

Methods in molecular biology, 1064-3745 ; 969
Springer protocols

ISBN:

1627032606 (eBook)
9781627032605 (eBook)
1627032592 (alk. paper)
9781627032599 (alk. paper)

OCLC:

(OCoLC)806020909

LCCN:

2012952730

Locations:

OVUX522 -- University of Iowa Libraries (Iowa City)