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03497aam a2200373 i 4500 001 AB410058580511E8A8F83C5097128E48 003 SILO 005 20180515010114 008 170808t20182018nyua b 001 0deng d 010 $a 2017959552 020 $a 9781493974580 020 $a 1493974580 035 $a (OCoLC)1000046502 040 $a YDX $b eng $c YDX $d NUI $d SILO 245 00 $a DNA topoisomerases : $b methods and protocols / $c edited by Marc Drolet. 264 1 $a New York, NY : $b Humana Press, $c [2018] 300 $a xi, 328 pages : $b illustrations (some color) ; $c 26 cm 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v volume 1703 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographical references and index. 505 0 $a Type IA DNA Topoisomerases: A Universal Core and Multiple Activities -- Topoisomerase I and Genome Stability: The Good and The Bad -- DNA Topoisomerases as Targets for Antibacterial Agents -- DNA Supercoiling Measurement in Bacteria -- DNA Catenation Reveals the Dynamics of DNA Topology during Replication -- Mapping E. coli Topoisomerase IV Binding and Activity Sites -- The Use of Psoralen Photobinding to Study Transcription-Induced Supercoiling -- Immunoprecipitation of RNA:DNA Hybrids from Budding Yeast -- Detection of oriC-Independent Replication in Escherichia coli Cells -- Single-Molecule Magnetic Tweezer Analysis of Topoisomerases -- Synthesis of Hemicatenanes for the Study of Type IA Topoisomerases -- An Assay for Detecting RNA Topoisomerase Activity -- Studying TDP1 Function in DNA Repair -- Topoisomerase II Chromatin Immunoprecipitation -- Analyzing Mitotic Chromosome Structural Defects after Topoisomerase II Inhibition or Mutation -- Monitoring the DNA Topoisomerase II Checkpoint in Saccharomyces cerevisiae -- Studying Topoisomerase 1-Mediated Damage at Genomic Ribonucleotides -- A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons -- Fluoroquinolone-Gyrase-DNA Cleaved Complexes -- Detection of Topoisomerase Covalent Complexes in Eukaryotic Cells -- Visualization and Quantification of Topoisomerase-DNA Covalent Complexes Using the Trapped in Agarose Immunostaining (TARDIS) Assay -- Study of Plasmid-Mediated Quinolone Resistance in Bacteria. 520 $a This volume looks at the evolution of the topo field over the last year and discusses how DNA topoisomerases are directly and indirectly involved in numerous cellular processes. The chapters in this book cover various topics, such as type 1 topos with an evolutionary perspective; antimicrobial agents targeting topos; experimental protocols related to DNA topology, including a basic technique to measure DNA supercoiling in bacteria; protocols for in vitro studies, including assays to detect RNA topo activity; topo inhibitors; and a study of plasmid-mediated quinolone resistance in bacteria. 650 0 $a DNA topoisomerases $v Laboratory manuals. 650 0 $a Human genetics. 650 7 $a DNA topoisomerases. $2 fast $0 (OCoLC)fst00886607 655 7 $a Laboratory manuals. $2 fast $0 (OCoLC)fst01920776 655 7 $a Laboratory manuals. $2 lcgft 700 1 $a Drolet, Marc, $e editor. 773 18 $w 990001282000202771 $g v:1703 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1703. $x 1064-3745 830 0 $a Springer protocols (Series), $x 1949-2448 941 $a 1 952 $l OVUX522 $d 20181116051916.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=AB410058580511E8A8F83C5097128E48Initiate Another SILO Locator Search