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Title:

Laboratory methods in enzymology. DNA / edited by Jon Lorsch.

Edition:

First edition.

Publisher:

Elsevier/Academic Press,

Copyright Date:

2013

Description:

lvi, 348 pages : illustrations ; 24 cm.

Subject:

Biotechnology--Methodology.
Molecular biology--Methodology.

Other Authors:

Lorsch, Jon, editor of compilation.

Notes:

Includes bibliographical references and indexes.

Contents:

Explanatory chapter: Transformation of chemically competent E. coli / Rachel Green and Elizabeth J. Rogers. Explanatory chapter: How plasmid preparation kits work / Laura Koontz -- Explanatory chapter: Introducing exogenous DNA into cells / Laura Koontz -- Agarose gel electrophoresis / Laura Koontz -- Analysis of DNA by southern blotting / Gary Glenn and Lefkothea-Vasiliki Andreou -- Purification of DNA oligos by denaturing polyacrylamide gel electrophoresis (PAGE) / Sara Lopez-Gomollon and Francisco Esteban Nicolas -- Molecular cloning / Juliane C. Lessard -- Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway℗ʼ recombination cloning technology and Flp-In T-REx℗ʼ lines / Jessica Spitzer, Markus Landthaler, and Thomas Tuschl -- Restrictionless cloning / Mikkel A. Algire -- Isolation of plasmid DNA from bacteria / Lefkothea-Vasiliki Andreou -- Preparation of genomic DNA from bacteria / Lefkothea-Vasiliki Andreou -- Preparation of genomic DNA from Saccharomyces cerevisiae / Jessica S. Dymond -- Isolation of genomic DNA from mammalian cells / Cheryl M. Koh -- Sanger dideoxy sequencing of DNA / Sarah E. Walker and Jon Lorsch -- Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform / Srinivasan Yegnasubramanian -- Explanatory chapter: Next generation sequencing / Srinivasan Yegnasubramanian -- Generating mammalian stable cell lines by electroporation / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- Transient mammalian cell transfection with polyethylenimine (PEI) / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- Site-directed mutagenesis / Julia Bachman -- PCR-based random mutagenesis / Jessica S. Dymond -- Megaprimer method for mutagenesis of DNA / Craig W. Vander Kooi -- Explanatory chapter: Troubleshooting PCR / Kirstie Canene-Adams -- Explanatory chapter: Quantitative PCR / Jessica S. Dymond -- General PCR / Kirstie Canene-Adams -- Colony PCR / Megan Bergkessel and Christine Guthrie -- Chemical transformation of yeast / Megan Bergkessel and Christine Guthrie -- Transformation of E. coli via electroporation / Juliane C. Lessard -- Transformation of chemically competent E. coli / Rachel Green and Elizabeth J. Rogers.

Series:

Methods in enzymology, 0076-6879 ; volume 529.

ISBN:

0124186874
9780124186873

OCLC:

(OCoLC)858970409

Locations:

OIAX792 -- Grinnell College (Grinnell)