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Title:

PCR protocols / edited by Daniel J. Park.

Edition:

3rd ed.

Publisher:

Humana Press,

Copyright Date:

c2011

Description:

xi, 348 p. : ill. ; 27 cm.

Subject:

Polymerase chain reaction--Laboratory manuals.
Polymerase Chain Reaction--methods--Laboratory Manuals.

Other Authors:

Park, Daniel J.

Notes:

Includes bibliographical references and index.

Contents:

Setup of a PCR laboratory / Zaheer Khan -- Long-range PCR with a DNA polymerase fusion / Holly H. Hogrefe and Michael C. Borns -- Isolation of genomic insertion sites of proviruses using splinkerette-PCR-based procedures / Bin Yin -- Lariat-dependent nested PCR for flanking sequence determination / Daniel J. Park -- CODEHOP PCR and CODEHOP PCR primer design / Jeannette Pl Staheli ... [et al.] -- Sequencing of difficult DNA regions by SAM sequencing / Keith R. Mitchelson -- A global single-cell cDNA amplification method for quantitative microarray analysis / Kazuki Kurimoto and Mitinori Saitou -- Quantitation of microRNAs by real-time RT-qPCR / Caifu Chen ... [et al.] -- High-throughput SuperSAGE / Hideo Matsumura ... [et al.] -- Deep cap analysis of gene expression / Junpei Kurosawa, Hiromi Nichiyori, and Yoshihide Hayashizaki -- Linking emulsion PCR haplotype analysis / James G. Wetmur and Jia Chen --
PAP-LMPCR : an improved, sequence-selective method for the in vivo analysis of transcription factor occupancy and chromatin fine structure / Richard ingram, Arthur Riggs, and Constanze Bonifer -- The many faces of MLPA / Thomas Ohnesorg, Erin Turbitt, and Stefan J. White -- Assessing gene-specific methylation using HRM-based analysis / Ee Ming Wong and Alexander Dobrovic -- Alu PCR / Maurizio Cardelli -- Asynchronous PCR / Caifu Chen, David Ruff, and Jason Halsey -- Novel applications of PCR through the use of DNA substrates / Stuart M. Wilson -- Enhanced solid phase PCR for increased loading of amplicon onto solid support / Daniel J. Park -- Application of blocking oligonucleotides to improve signal-to-noise ratio in a PCR / Hege Vestheim, Bruce E. Deagle, and Simon N. Jarman -- Asymmetric overlap extension PCR method for site-directed mutagenesis / Yue-Hua Xiao and Yan Pei --
Ribosome display : a technology for selecting and evolving proteins from large libraries / Birgit Dreier and Andreas Plückthun -- GLOBE : analysis of DNA-protein interaction analysis / Takaaki Kojima and Hideo Nakano -- PCR DNA-array profiling of DNA-binding transcriptin factor activities in adult mouse tissues / Yimin Sun, Jing Cheng, and Keith R. Mitchelson -- Nucleotide exchange and excision technology DNA shuffling and directed evolution / Janina Speck ... [et al.].

Series:

Springer protocols
Methods in molecular biology, 1064-3745 ; 687

ISBN:

1607619431 (alk. paper)
9781607619437 (alk. paper)

OCLC:

(OCoLC)648933277

Locations:

OVUX522 -- University of Iowa Libraries (Iowa City)