Qualitative and quantitative assays for flagellum- mediated chemotaxis / José Antonio Reyes Darias [and five others] -- Gene transfer : transformation/electroporation / Frédéric Cadoret, Chantal Soscia, and Romé Voulhoux -- Gene transfer : conjugation / Thibault G. Sana, Aurélie Laubier, and Sophie Bleves -- Pseudomonas bacteriophage isolation and production / Joana Azeredo, Sanna Sillankorva, and Diana P. Pires -- Genotyping methods / Burkhard Tümmler -- Drug susceptibility testing by dilution methods / Katy Jeannot and Patrick Plésiat -- Plate-based assay for swimming motility in Pseudomonas aeruginosa / Dae-Gon Ha, Sherry L. Kuchma, and George A. O'Toole -- Plate-based assay for swarming motility in Pseudomonas aeruginosa / Dae-Gon Ha, Sherry L. Kuchma, and George A. O'Toole -- Motility assay : twitching motility / Lynne Turnbull and Cynthia B. Whitchurch -- Qualitative and quantitative assays for flagellum- mediated chemotaxis / José Antonio Reyes Darias [and five others] -- Construction of Pseudomonas aeruginosa two-hybrid libraries for high-throughput assays / Sophie de Bentzmann and Christophe Bordi -- Determination of lipolytic enzyme activities / Karl-Erich Jaeger and Filip Kovacic -- Elastinolytic and proteolytic enzymes / Efrat Kessler and Mary Safrin -- In vitro assays to monitor the activity of Pseudomonas aeruginosa type III secreted proteins / Stephanie L. Rolsma and Dara W. Frank -- Cell fractionation / Bérengère Ize, Véronique Viarre, and Romé Voulhoux -- Characterization of molecular interactions using isothermal titration calorimetry / Tino Krell [and seven others] -- Proteomic analysis / Pascal Cosette and Thierry Jouenne -- Membrane proteomics of Pseudomonas aeruginosa / Zofia Magnowska, Isabel Hartmann, Lothar Jänsch, and Dieter Jahn -- Construction of Pseudomonas aeruginosa two-hybrid libraries for high-throughput assays / Sophie de Bentzmann and Christophe Bordi -- Monitoring iron uptake by siderophores / Chandrika Goh Nair, Ben Ryall, and Huw D. Williams -- LC-MS/MS quantitative analysis of quorum sensing signal molecules / Catharine A. Ortori [and four others] -- LC/MS/MS-based quantitative assay for the secondary messenger molecule, c-di-GMP / Yasuhiko Irie and Matthew R. Parsek -- Metabolic footprinting : extracellular metabolomic analysis / Volker Behrends, Huw D. Williams, and Jacob G. Bundy -- Pyoverdine and pochelin measurements / Françoise Hoegy, Gaetan L. A. Mislin, and Isabelle J. Schalk -- Measurement of phenazines in bacterial cultures / Suzanne E. Kern and Dianne K. Newman -- Extraction and measurement of NAD(P)+ and NAD(P)H / Suzanne E. Kern, Alexa Price-Whelan, and Dianne K. Newman -- Cyanide measurements in bacterial culture and sputum / Chandrika Goh Nair, Ben Ryall, and Huw D. Williams -- Monitoring iron uptake by siderophores / Rafael Silva-Rocha and Víctor de Lorenzo -- Chromosomal integration of transcriptional fusions / Irina Sadovskaya -- Liquid chromatography/mass spectrometry for the identification and quantification of rhamnolipids / Ahmad Mohammad Abdel-Mawgoud, François Lépine, and Eric Déziel -- LPS quantitation procedures / Joseph S. Lam, Erin M. Anderson, and Youai Hao -- Monitoring lectin interactions with carbohydrates / Sophie de Bentzmann, Annabelle Varrot, and Anne Imberty -- Mining the Pseudomonas genome / Geoffrey L. Winsor and Fiona S. L. Brinkman -- Identification of bacterial small RNAs by RNA sequencing / María Gómez-Lozano [and three others.] -- Gene amplification and qRT-PCR / Cerith Jones and Alain Filloux -- The Standard European Vector Architecture (SEVA) plasmid toolkit / Gonzalo Durante-Rodríguez, Víctor de Lorenzo, and Esteban Martínez-García -- Chromosomal integration of transcriptional fusions / Rafael Silva-Rocha and Víctor de Lorenzo -- Transcriptional analysis of Pseudomonas aeruginosa infected Caenorhabditis elegans / Ashleigh MacKenzie [and three others] -- Transposon mutagenesis / Hemantha D. Kulasekara -- Site-directed mutagenesis and gene deletion using reverse genetics / Daniela Muhl and Alain Filloux -- Signature-tagged mutagenesis / Irena Kukavica-Ibrulj and Roger C. Levesque -- Construction of a Pseudomonas aeruginosa genomic DNA library / Christophe Bordi -- Strategy for genome sequencing analysis and assembly for comparative genomics of Pseudomonas genomes / Julie Jeukens [and three others] -- Promoter fusions with optical outputs in individual cells and in populations / Ilaria Benedetti and Victor de Lorenzo -- Chromatin immunoprecipitation for ChIP-chip and ChIP-seq / Sebastian Schulz and Susanne Häussler -- Transcriptional analysis of Pseudomonas aeruginosa infected Caenorhabditis elegans / Ashleigh MacKenzie [and three others] -- Assessing Pseudomonas aeruginosa persister/antibiotic tolerant cells / Ronen Hazan [and three others] -- Biofilm formation in the 96-well microtiter plate / Barbara M. Coffey and Gregory G. Anderson -- Methods for studying biofilm dispersal in Pseudomonas aeruginosa / Nicolas Barraud [and three others] -- Pseudomonas aeruginosa PA14 pathogenesis in Caenorhabditis elegans / Natalia V. Kirienko [and three others] -- Assessing Pseudomonas aeruginosa virulence using a nonmammalian host : Dictyostelium discoideum / Geneviève Filion and Steve J. Charette -- Assessing Pseudomonas virulence with nonmammalian host : Galleria mellonella / Gudrun Koch [and three others] -- Assessing Pseudomonas virulence with the nonmammalian host model : Arabidopsis thaliana / Regina L. Baldini, Melissa Starkey, and Laurence G. Rahme -- Assessing Pseudomonas aeruginosa persister/antibiotic tolerant cells / Ronen Hazan [and three others] -- Burn mouse models / Henrik Calum, Niels Høiby, and Claus Moser. Assessing Pseudomonas virulence with a nonmammalian host : Drosophila melanogaster / Samantha Haller, Stefanie Limmer, and Dominique Ferrandon -- Assessing Pseudomonas virulence using host cells / Iwona Bucior, Cindy Tran, and Joanne Engel -- Assessing Pseudomonas aeruginosa virulence and the host response using murine models of acute and chronic lung infection / Irena Kukavica-Ibrulj [and four others] -- Assessing Pseudomonas virulence using mammalian models : acute infection model / Antje Munder and Burkhard Tümmler -- Burn mouse models / Henrik Calum, Niels Høiby, and Claus Moser.
Summary:
"In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings."--Back cover.
Series:
Methods in Molecular Biology, 1064-3745 ; 1149 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.