Extracting knowledge from MS clinical metabolomic data : processing and analysis strategies / Julien Boccard and Serge Rudaz. Metabolomics in immunology research / Bart Everts -- LC-MS-based metabolomics of biofluids using all-ion fragmentation (AIF) acquisition / Romanas Chaleckis, Shama Naz, Isabel Meister, and Craig E. Wheelock -- Lipid mediator metabolomics via LC-MS/MS profiling and analysis / Jesmond Dalli, Romain A. Colas, Mary E. Walker, and Charles N. Serhan -- UHPSFC/ESI-MS analysis of lipids / Miroslav Lisa and Michal Holcapek -- LC-MS/MS analysis of lipid oxidation products in blood and tissue samples / Yiu Yiu Lee and Jetty Chung-Yung Lee -- Serum testosterone by liquid chromatography tandem mass spectrometry for routine clinical diagnostics / Lennart J. van Winden, Olaf van Tellingen, and Huub H. van Rossum -- LC-MS/MS analysis of bile acids / Sabrina Krautbauer and Gerhard Liebisch -- LC-MS/MS analysis of triglycerides in blood-derived samples / Madlen Reinicke, Susen Becker, and Uta Ceglarek -- LC-MS/MS analysis of the epoxides and diols derived from the endocannabinoid arachidonoyl ethanolamide / Amy A. Rand, Patrick O. Helmer, Bora Inceoglu, Bruce D. Hammock, and Christophe Morisseau -- Sphingolipid analysis in clinical research / Bo Burla, Sneha Muralidharan, Markus R. Wenk, and Federico Torta -- Shotgun lipidomics approach for clinical samples / Lars F. Eggers and Dominik Schwudke -- Establishing and performing targeted multi-residue analysis for lipid mediators and fatty acids in small clinical plasma samples / Theresa L. Pedersen and John W. Newman -- Chemical isotope labeling LC-MS for human blood metabolome analysis / Wei Han and Liang Li -- Direct infusion-tandem mass spectrometry (DI-MS/MS) analysis of complex lipids in human plasma and serum using the lipidyzer platform / Baljit K. Ubhi -- Exploratory GC/MS-based metabolomics of body fluids / Carole Migne, Stephanie Durand, and Estelle Pujos-Guillot -- GC-MS analysis of short-chain fatty acids in feces, cecum content, and blood samples / Lisa R. Hoving, Marieke Heijink, Vanessa van Harmelen, Ko Willems van Dijk, and Martin Giera -- GC-MS analysis of medium- and long-chain fatty acids in blood samples / Lisa R. Hoving, Marieke Heijink, Vanessa van Harmelen, Ko Willems van Dijk, and Martin Giera -- Analysis of oxysterols / Fabien Riols and Justine Bertrand-Michel -- Analysis of metabolites from the tricarboxylic acid cycle for yeast and bacteria samples using gas chromatography mass spectrometry / Reza Maleki Seifar, Angela ten Pierick, and Patricia T.N. van Dam -- GC-MS analysis of lipid oxidation products in blood, urine, and tissue samples / Anne Barden and Trevor A. Mori -- Metabolic profiling of urine by capillary electrophoresis-mass spectrometry using non-covalently coated capillaries / Rawi Ramautar -- CE-MS for the analysis of amino acids / Karina Trevisan Rodrigues, Marina Franco Maggi Tavares, and Ann Van Schepdael -- NMR analysis of fecal samples / Hye Kyong Kim, Sarantos Kostidis, and Young Hae Choi -- Quantitative analysis of central energy metabolism in cell culture samples / Sarantos Kostidis -- Mass spectrometry imaging of metabolites / Benjamin Balluff and Liam A. McDonnell -- Quality-assured biobanking : the Leiden University Medical Center model / Rianne Haumann and Hein W. Verspaget -- Extracting knowledge from MS clinical metabolomic data : processing and analysis strategies / Julien Boccard and Serge Rudaz.
Summary:
This detailed volume presents a comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. The methodologies explored here form the core of several very promising initiatives evolving around personalized health care and precision medicine, which can be seen as complimentary to the field of clinical chemistry and aid the aforementioned field with novel disease markers and diagnostic patterns. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.uthoritative and practical, Clinical Metabolomics: Methods and Protocols aims to serve as the basis for successful communication between scientists from several fields, including chemists, biologist, bioinformaticians, and clinicians, ultimately leading to effective study design and completion.
Series:
Methods in molecular biology, 1064-3745 ; 1730 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.