Visualization of fission yeast cells by transmission electron microscopy / Matthias Sipiczki -- Real-time visualization and quantification of contractile ring proteins in single living cells / Reshma Davidson [and others] -- Fluorescence recovery after photo-bleaching (FRAP) and fluorescence loss in photo-bleaching (FLIP) experiments to study protein dynamics during budding yeast cell division / Alessio Bolognesi [and others] -- High-speed super-resolution imaging of live fission yeast cells / Caroline Laplante [and others] -- Monitoring chitin deposition during septum assembly in budding yeast / Irene Arcones and Cesar Roncero -- Imaging septum formation by fluorescence microscopy / Juan Carlos Ribas and Juan Carlos G. Cortés -- Visualization of cytokinesis events in budding yeast by transmission electron microscopy / Franz Meitinger and Gislene Pereira -- Visualization of fission yeast cells by transmission electron microscopy / Matthias Sipiczki -- Detection of phosphorylation status of cytokinetic components / Franz Meitinger, Saravanan Palani, and Gislene Pereira -- Isolation of cytokinetic actomyosin rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe / Junqi Huang [and others] -- Measurements of myosin-II motor activity during cytokinesis in fission yeast / Qing Tang, Luther W. Pollard, and Matthew Lord -- In vitro biochemical characterization of cytokinesis actin-binding proteins / Dennis Zimmermann [and others] -- Characterization of cytokinetic F-BARs and other membrane-binding proteins / Nathan A. McDonald and Kathleen L. Gould -- Analysis of three-dimensional structures of exocyst components / Johannes Lesigang and Gang Dong -- Analysis of Rho-GTPase activity during budding yeast cytokinesis / Masayuki Onishi and John R. Pringle -- Detection of phosphorylation status of cytokinetic components / Franz Meitinger, Saravanan Palani, and Gislene Pereira -- From structure to function : a comprehensive compendium of tools to unveil protein domains and understand their role in cytokinesis / Daniel Barry Roche and Liam James McGuffin -- Conditional budding yeast mutants with temperature-sensitive and auxin-inducible degrons for screening of suppressor genes / Asli Devrekanli and Masato T. Kanemaki -- Synchronization of the budding yeast Saccharomyces cerevisiae / Magdalena Foltman, Iago Molist, and Alberto Sanchez-Diaz -- Fission yeast cell cycle synchronization methods / Marta Tormos-Pérez, Livia Pérez-Hidalgo, and Sergio Moreno -- Review of fluorescent proteins for use in yeast / Maja Bialecka-Fornal, Tatyana Makushok, and Susanne M. Rafelski -- Visualization and image analysis of yeast cells / Steve Bagley -- Toolbox for protein structure prediction / Daniel Barry Roche and Liam James McGuffin -- From structure to function : a comprehensive compendium of tools to unveil protein domains and understand their role in cytokinesis / Sergio A. Rincon and Anne Paoletti.
Summary:
This volume presents protocols on yeast cytokinesis that will help Molecular and Cellular Biology researchers in the use of these microorganisms to approach the study of general or specific key questions in cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Cytokinesis: Methods and Protocols provides practical and step-by-step detailed protocols useful for a wide audience ranging from experienced researchers to beginners in the use of yeasts.
Series:
Methods in molecular biology, 1064-3745 ; 1369 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.