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Title:
Transposons and retrotransposons : methods and protocols / edited by Jose L. Garcia-Pérez.
Publisher:
Humana Press,
Copyright Date:
2016
Description:
xii, 434 pages : illustrations (some color) ; 27 cm.
Subject:
Transposons--Research.
DNA Transposable Elements.
Retroelements.
Laboratory Manuals.
Other Authors:
García Pérez, José L. (Geneticist), editor. http://id.loc.gov/authorities/names/nb2016006987
Notes:
Includes bibliographical references and index.
Contents:
RNA-Seq analysis to measure the expression of SINE retroelements / Ángel Carlos Román, Antonio Morales-Hernández, and Pedro M. Fernández-Salguero -- Bacterial group II introns : identification and mobility assay / Nicolás Toro ... [et al.] -- In silico methods to identify exapted transposable element families / LeeAnn Ramsay and Guillaume Bourque -- Retrotransposon capture sequencing (RC-Seq) : a targeted, high-throughput approach to resolve somatic L1 retrotransposition in humans / Francisco J. Sanchez-Luque, Sandra R. Richardson, and Geoffrey J. Faulkner -- Long interspersed element sequencing (L1-Seq) : a method to identify somatic LINE-1 insertions in the human genome / Tara T. Doucet and Haig H. Kazazian Jr. -- Combining amplification typing of L1 active subfamilies (ATLAS) with high-throughput sequencing / Raheleh Rahbari and Richard M. Badge -- RNA-Seq analysis to measure the expression of SINE retroelements / Ángel Carlos Román, Antonio Morales-Hernández, and Pedro M. Fernández-Salguero --
Monitoring long interspersed nuclear element 1 expression during mouse embryonic stem cell differentiation / Prescott Deininger and Victoria P. Belancio -- LINE retrotransposition assays in Saccharomyces cerevisiae / Axel V. Horn and Jeffrey S. Han -- LINE-1 cultured cell retrotransposition assay / Huira C. Kopera ...[et al.] -- L1 retrotransposition in neural progenitor cells / Alysson R. Muotri -- Characterization of engineered L1 retrotransposition events : the recovery method / David Cano ... [et al.] -- SINE retrotransposition : evaluation of alu activity and recovery of de novo inserts / Catherine Ade and Astrid M. Roy-Engel -- Engineered SVA trans-mobilization assay / Anja Bock and Gerald G. Schumann -- Detection of LINE-1 RNAs by northern blot / Prescott Deininger and Victoria P. Belancio -- Monitoring long interspersed nuclear element 1 expression during mouse embryonic stem cell differentiation /
Rita Rebollo and Dixie L. Mager -- Profiling DNA methylation and hydroxymethylation at retrotransposable elements / Reema Sharma ... [et al.] -- Cellular localization of engineered human LINE-1 RNA and proteins / Aurélien J. Doucet, Eugénia Basyuk, and Nicolas Gilbert -- Purification of L1-ribonucleoprotein particles (L1-RNPs) from cultured human cells / Prabhat K. Mandal and Haig H. Kazazian Jr. -- Characterization of L1-ribonucleoprotein particles / Martin S. Taylor ... [et al.] -- LEAP : L1 element amplification protocol / Huira C. Kopera ... [et al.] -- Biochemical approaches to study LINE-1 reverse transcriptase activity in vitro / Sébastien Viollet, Aurélien J. Doucet, and Gaël Cristofari -- Methylated DNA immunoprecipitation analysis of mammalian endogenous retroviruses / Rita Rebollo and Dixie L. Mager -- Profiling DNA methylation and hydroxymethylation at retrotransposable elements /
Attila Sebe and Zoltán Ivics. Reprogramming of human fibroblasts to induced pluripotent stem cells with sleeping beauty transposon-based stable gene delivery / Gabriela Ecco, Helen M. Rowe, and Didier Trono -- Reprogramming of human fibroblasts to induced pluripotent stem cells with sleeping beauty transposon-based stable gene delivery / Attila Sebe and Zoltán Ivics.
Series:
Methods in molecular biology, 1064-3745 ; 1400
Springer protocols, 1949-2448
ISBN:
9781493933709
1493933701
OCLC:
(OCoLC)945638115
LCCN:
2015960958
Locations:
OVUX522 -- University of Iowa Libraries (Iowa City)

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