10 records matched your query
03458aam a2200301Ii 4500 001 364EAC163E3011E4861469EFDAD10320 003 SILO 005 20140917010101 008 130927s2013 ne a b 001 0 eng d 020 $a 0124186874 020 $a 9780124186873 035 $a (OCoLC)858970409 040 $a LHL $e rda $c LHL $d OCLCO $d UPP $d AAA $d DRU $d YDXCP $d UKMGB $d SILO 245 00 $a Laboratory methods in enzymology. $p DNA / $c edited by Jon Lorsch. 246 30 $a DNA. 250 $a First edition. 264 1 $a Amsterdam : $b Elsevier/Academic Press, $c 2013. 300 $a lvi, 348 pages : $b illustrations ; $c 24 cm. 490 1 $a Methods in enzymology, $x 0076-6879 ; $v volume 529. 504 $a Includes bibliographical references and indexes. 505 00 $g Explanatory chapter: $t Transformation of chemically competent E. coli / $r Rachel Green and Elizabeth J. Rogers. $g Explanatory chapter: $t How plasmid preparation kits work / $r Laura Koontz -- $g Explanatory chapter: $t Introducing exogenous DNA into cells / $r Laura Koontz -- $t Agarose gel electrophoresis / $r Laura Koontz -- $t Analysis of DNA by southern blotting / $r Gary Glenn and Lefkothea-Vasiliki Andreou -- $t Purification of DNA oligos by denaturing polyacrylamide gel electrophoresis (PAGE) / $r Sara Lopez-Gomollon and Francisco Esteban Nicolas -- $t Molecular cloning / $r Juliane C. Lessard -- $t Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gatewayâʼ recombination cloning technology and Flp-In T-RExâʼ lines / $r Jessica Spitzer, Markus Landthaler, and Thomas Tuschl -- $t Restrictionless cloning / $r Mikkel A. Algire -- $t Isolation of plasmid DNA from bacteria / $r Lefkothea-Vasiliki Andreou -- $t Preparation of genomic DNA from bacteria / $r Lefkothea-Vasiliki Andreou -- Preparation of genomic DNA from Saccharomyces cerevisiae / $r Jessica S. Dymond -- $t Isolation of genomic DNA from mammalian cells / $r Cheryl M. Koh -- $t Sanger dideoxy sequencing of DNA / $r Sarah E. Walker and Jon Lorsch -- $t Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform / $r Srinivasan Yegnasubramanian -- $g Explanatory chapter: $t Next generation sequencing / $r Srinivasan Yegnasubramanian -- $t Generating mammalian stable cell lines by electroporation / $r Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- $t Transient mammalian cell transfection with polyethylenimine (PEI) / $r Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- $t Site-directed mutagenesis / $r Julia Bachman -- $t PCR-based random mutagenesis / $r Jessica S. Dymond -- $t Megaprimer method for mutagenesis of DNA / $r Craig W. Vander Kooi -- $g Explanatory chapter: $t Troubleshooting PCR / $r Kirstie Canene-Adams -- $g Explanatory chapter: $t Quantitative PCR / Jessica S. Dymond -- $t General PCR / $r Kirstie Canene-Adams -- $t Colony PCR / $r Megan Bergkessel and Christine Guthrie -- $t Chemical transformation of yeast / $r Megan Bergkessel and Christine Guthrie -- $t Transformation of E. coli via electroporation / $r Juliane C. Lessard -- $t Transformation of chemically competent E. coli / $r Rachel Green and Elizabeth J. Rogers. 650 0 $a Biotechnology $x Methodology. 650 0 $a Molecular biology $x Methodology. 700 1 $a Lorsch, Jon, $e editor of compilation. 830 0 $a Methods in enzymology ; $v v. 529. 941 $a 1 952 $l OIAX792 $d 20140917012742.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=364EAC163E3011E4861469EFDAD10320Initiate Another SILO Locator Search