Isolation of human mesenchymal stem cells for studying ErbB receptor signaling / Chao Chen and Hongxing Jiang. New insights from drosophila into the regulation of EGFR signaling / Nicholas Harden -- C. elegans vulva induction : an in vivo model to study epidermal growth factor receptor signaling and trafficking / Kimberley Gauthier and Christian E. Rocheleau -- Targeting HER2 in advanced breast cancer / Xiaofu Zhu and Anil Abraham Joy -- Methods to investigate EGFR ubiquitination / Alexia Conte and Sara Sigismund -- Dimerization assessment of epithelial growth factor family of receptor tyrosine kinases by using cross-linking reagent / Hamid Maadi, Babak Nami, and Zhixiang Wang -- Application of immunofluorescence staining to study ErbB family of receptor tyrosine kinases / Babak Nami and Zhixiang Wang -- Activation of endosome-associated inert EGF receptor following internalization / Yi Wang, Sukhmani Billing, and Zhixiang Wang -- Two-pulse endosomal stimulation of receptor tyrosine kinases induces cell proliferation / Steven Pennock, Sukhmani Billing, Zhixiang Wang, and Yi Wang -- Study of EGFR signaling/endocytosis by site-directed mutagenesis / Qian Wang and Zhixiang Wang -- Using percoll gradient fractionation to study the endocytic trafficking of the EGFR / Julie A. Gosney and Brian P. Ceresa -- Analysis of epidermal growth factor receptor-induced cell motility by wound healing assay / Junfeng Tong and Zhixiang Wang -- Cell cycle synchronization of HeLa cells to assay EGFR pathway activation / Ping Wee and Zhixiang Wang -- Analysis of constitutive EGFR signaling regulating IRF3 transcriptional activity in cancer cells / Gao Guo, Ke Gong, and Amyn A. Habib -- Measurement of epidermal growth factor receptor-derived signals within plasma membrane clathrin structures / Stefanie Lucarelli, Ralph Christian Delos Santos, and Costin N. Antonescu -- Studying nonproliferative roles for Egfr signaling in tissue morphogenesis using dorsal closure of the drosophila embryo / Bruce Reed and Nicholas Harden -- Analysis of epithelial-mesenchymal transition induced by overexpression of twist / Jing-Wen Bai, Yong-Qu Zhang, Yao-Chen Li, and Guo-Jun Zhang -- Assessment of specificity of an adenovirus targeted to HER3/4 / Sheena H. MacLeod, Kyle G. Potts, Shyambabu Chaurasiya, and Mary M. Hitt -- Isolation of human mesenchymal stem cells for studying ErbB receptor signaling / Chao Chen and Hongxing Jiang.
Summary:
This volume contains protocols specifically designed for studying cell signaling mediated by ErbB receptors, all applicable to the study of a broad range of ErbB receptor-mediated signaling from basic research to clinic applications, from cultured cells to various animal models and primary cancer cells from patients. After some overviews, the book continues by covering common protocols for studying various aspects of ErbB receptor-mediated cell signaling, newly-developed methods in biomedical research that are also widely-used in the study of ErbB receptor signaling, epidermal growth factor receptor (EGFR) signaling in Drosophila, as well as protocols for studying ErbB receptor signaling in various animal model systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Detailed and easy-to-follow, ErbB Receptor Signaling: Methods and Protocols serves as an ideal guide for researchers who are studying the cell signaling mediated by ErbB receptors and other receptor tyrosine kinases.
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.