Quantification of neurotransmitters from intact and regenerating planarians using UHPLC-MS/SRM method / Kannan Rangiah and Dasaradhi Palakodeti. Stem cells, patterning and regeneration in planarians : self-organization at the organismal scale / Jochen C. Rink -- Ecology of freshwater planarians / Miquel Vila-Farré and Jochen C. Rink -- Planarians and the history of animal regeneration : paradigm shifts and key concepts in biology / Sarah A. Elliott and Alejandro Sánchez Alvarado -- Culturing planarians in the laboratory / M. Shane Merryman, Alejandro Sánchez Alvarado, and James C. Jenkin -- Total RNA isolation from planarian tissues / Shang-Yun Liu and Jochen C. Rink -- Small- and large-scale high molecular weight genomic DNA extraction from planarians / Markus A. Grohme, Miquel Vila-Farré, and Jochen C. Rink -- Planarian high molecular weight DNA isolation by spooling / Shasha Zhang and Alejandro Sánchez Alvarado -- Basic histological techniques for planarians / Leigh Winsor and Ronald Sluys -- Fixation, processing, and immunofluorescent labeling of whole mount planarians / David J. Forsthoefel, Kelly G. Ross, Phillip A. Newmark, and Ricardo M. Zayas -- Immunohistochemistry on paraffin-embedded planarian tissue sections / Teresa Adell, Sara Barberán, Miquel Sureda-Gómez, María Almuedo-Castillo, Nidia de Sousa, and Francesc Cebrià -- Whole-mount in situ hybridization of planarians / Ryan S. King and Phillip A. Newmark -- RNA in situ hybridization on planarian paraffin sections / Jordi Solana -- General principles of planarian embryogenesis and its analysis by in situ hybridization and immunohistochemistry methods / José María Martín-Durán -- Whole-mount BrdU staining with fluorescence in situ hybridization in planarians / Li-Chun Cheng and Alejandro Sánchez Alvarado -- Detection of apoptotic cells in planarians by whole-mount TUNEL / Brad Stubenhaus and Jason Pellettieri -- Systemic RNA interference in planarians by feeding of dsRNA containing bacteria / Carolyn E. Adler and Alejandro Sánchez Alvarado -- RNA interference in planarians : feeding and injection of synthetic dsRNA / Norito Shibata and Kiyokazu Agata -- Subtractive FACS method for isolation of planarian stem cells and neural cells / Tetsutaro Hayashi and Kiyokazu Agata -- Clonal analysis of planarian stem cells by subtotal irradiation and single-cell transplantation / Irving E. Wang, Daniel E. Wagner, and Peter W. Reddien -- Tissue transplantations in planarians / José I. Rojo-Laguna and Emili Saló -- Live imaging of planaria / Wei Shen, Yun Shen, Yun Wah Lam, and Danny Chan -- Processing Schmidtea mediterranea for transmission electron microscopy : classical and microwave techniques / John L. Brubacher, Ana P. Vieira, and Juliette Azimzadeh -- Tissue extracts for quantitative mass spectrometry of planarian proteins using SILAC / Alexander Böser, Hannes C. A. Drexler, and Kerstin Bartscherer -- Quantification of neurotransmitters from intact and regenerating planarians using UHPLC-MS/SRM method / Kannan Rangiah and Dasaradhi Palakodeti.
Summary:
This volume explores the various facets of planaria as a biomedical model system and discusses techniques used to study the fascinating biology of these animals. The chapters in this book are divided into two parts: Part One looks at the biodiversity of planarian species, the molecular orchestration of regeneration, ecology of planarians in their natural habitats and their history as lab models. Part Two talks about experimental protocols for studying planarians, ranging from the establishment of a planarian research colony, to RNA and DNA extraction techniques, all the way to single stem cell transplantations or metabolomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Planarian Regeneration: Methods and Protocols is a valuable resource for both newcomers to the field and experts within established planarian laboratories.
Series:
Methods in molecular biology, 1064-3745 ; 1774 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.