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Title:
Auditory and vestibular research : methods and protocols / edited by Bernd Sokolowski.
Publisher:
Humana,
Copyright Date:
c2009
Description:
xii, 421 p. : ill. (some col.) ; 27 cm.
Subject:
Vestibular apparatus.
Hearing disorders.
Equilibrium (Physiology)
Auditory Pathways--physiology.
Ear Diseases--diagnosis.
Vestibule, Labyrinth--physiology.
Vestibular Diseases--diagnosis.
Vestibule du labyrinthe osseux.
Oreille interne.
Other Authors:
Sokolowski, Bernd.
Notes:
Includes bibliographical references and index.
Contents:
RNA isolation from Xenopus inner ear sensory endorgans for transcriptional profiling and molecular cloning / Casilda Trujillo-Provencio ... [et al.] -- Synthesis of biotin-labeled RNA for gene expression measurements using oligonucleotide arrays / Ana E. Vázquez, Liping Nie, and Ebenezer N. Yamoah -- In situ hybridization approach to study mRNA expression and distribution in cochlear frozen sections / Hakim Hiel -- Lineage analysis of inner ear cells using genomic tags for clonal identification / Takunori Satoh and Donna M. Fekete -- Genetic fate-mapping approaches : new means to explore the embryonic origins of the cochlear nucleus / Jun Chul Kim and Susan M. Dymecki -- The practical use of Cre and loxP technologies in mouse auditory research / Yiling Yu and Jian Zuo -- Helios gene gun-mediated transfection of the inner ear sensory epithelium / Inna A. Belyantseva -- Electroporation-mediated gene transfer to the developing mouse inner ear / John V. Brigande ... [et al.] -- Isolation of sphere-forming stem cells from the mouse inner ear / Kazuo Oshima, Pascal Senn, and Stefan Heller -- Molecular biology of vestibular schwannomas / Long-Sheng Chang and D. Bradley Welling -- Multilocus sequence typing and pulsed field gel electrophoresis of otitis media causing pathogens / Jonathan C. Thomas and Melinda M. Pettigrew -- Fluorescence "in situ" hybridization for the detection of biofilm in the middle ear and upper respiratory tract mucosa / Laura Nistico ... [et al.] -- Positional cloning of deafness genes / Hannie Kremer and Frans P.M. Cremers -- Twist-off purification of hair bundles / Jung-Bum Shin, James Pagana, and Peter G. Gillespie -- Yeast two-hybrid screening to test for protein-protein interactions in the auditory system / Dhasakumar S. Navaratnam -- The use of 2-D gels to identify novel protein-protein interactions in the cochlea / Thandavarayan Kathiresan, Margaret C. Harvey, and Bernd H.A. Sokolowski -- Identification of functionally important residues/domains in membrane proteins using an evolutionary approach coupled with systematic mutational analysis / Lavanya Rajagopalan ... [et al.] -- In vivo verification of protein interactions in the inner ear by coimmunoprecipitation / Margaret C. Harvey and Bernd H.A. Sokolowski -- Identification of transcription factor-DNA interactions using chromatin immunoprecipitation assays / Liping Nie, Ana E. Vázquez, and Ebenezer N. Yamoah -- Surface plasmon resonance (SPR) analysis of binding interactions of proteins in inner-ear sensory epithelia / Dennis G. Drescher, Neeliyath A. Ramakrishnan, Marian J. Drescher -- Multiplexed isobaric tagging protocols for quantitative mass spectrometry approaches to auditory research / Douglas E. Vetter, Johnvesly Basappa, and Sevin Turcan -- Fluorescence microscopy methods in the study of protein structure and function / Heather Jensen-Smith ... [et al.] -- Ion imaging in the cochlear hair cells / Gregory I. Frolenkov -- Atomic force microscopy in studies of the cochlea / Michio Murakoshi and Hiroshi Wada.
Series:
Springer protocols
Methods in molecular biology, 1064-3745 ; 493
ISBN:
1597455237 (ebk.)
9781597455237 (ebk.)
1934115622 (hbk.)
9781934115626 (hbk.)
OCLC:
(OCoLC)233933530
LCCN:
2008934544
Locations:
USUX851 -- Iowa State University - Parks Library (Ames)
OVUX522 -- University of Iowa Libraries (Iowa City)

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