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05937aam a2200421 i 4500 001 9436D1F8580511E8A8F83C5097128E48 003 SILO 005 20180515010114 008 170513t20172017nyua b 000 0deng d 010 $a 2017949390 020 $a 1493972308 020 $a 9781493972302 035 $a (OCoLC)1014041813 040 $a STF $b eng $c STF $d OCLCO $d GZM $d I8M $d OCLCF $d NUI $d SILO 245 00 $a Functional genomics : $b methods and protocols / $c edited by Michael Kaufmann, Claudia Klinger and Andreas Savelsbergh. 250 $a Third edition. 264 1 $a New York, NY : $b Humana Press ; $c [2017] 300 $a xv, 392 pages : $b illustrations (some color) ; $c 27 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 1654 490 1 $a Springer protocols, $x 1949-2478 504 $a Includes bibliographical references and index. 505 00 $t Production, purification, and titration of first-generation adenovirus vectors / $r Ramona F. Kratzer and Florian Kreppel. $t RNA function prediction / $r Yongsheng Li, Juan Xu, Tingting Shao, Yunpeng Zhang, Hong Chen, and Xia Li -- $t Computational prediction of novel miRNAs from genome-wide data / $r Georgina Stegmayer, Cristian Yones, Laura Kamenetzky, Natalia Macchiaroli, and Diego H. Milone -- $t Protein structure modeling with MODELLER / $r Benjamin Webb and Andrej Sali -- $t Protein function prediction / $r Leonardo Magalhaes Cruz, Sheyla Trefflich, Vinicius Almir Weiss, and Mauro Antonio Alves Castro -- $t Capturing three-dimensional genome organization in individual cells by single-cell hi-c / $r Takashi Nagano, Steven W. Wingett, and Peter Fraser -- $t Genome-wide cell type-specific mapping of in vivo chromatin protein binding using an FLP-inducible DamID system in Drosophila / $r Alexey V. Pindyurin -- $t DNA methylation profiling using long-read single molecule real-time bisulfite sequencing (SMRT-BS) / $r Yao Yang and Stuart A. Scott -- $t Copy number variation analysis by droplet digital PCR / $r Suvi K. Hâ¬armâ¬alâ¬a, Robert Butcher, and Chrissy H. Roberts -- $t MicroScale thermophoresis : a rapid and precise method to quantify protein-nucleic acid interactions in solution / $r Adrian Michael Mueller, Dennis Breitsprecher, Stefan Duhr, Philipp Baaske, Thomas Schubert, and Gernot Lâ¬angst -- $t Establishment of the CRISPR/Cas9 system for targeted gene disruption and gene tagging / $r Eric Ehrke-Schulz, Maren Schiwon, Claudia Hagedorn, and Anja Ehrhardt -- $t Holistic and affordable analyses of MicroRNA expression profiles using tagged cDNA libraries and a multiplex sequencing strategy / $r Patrick P. Weil, Yan Jaszczyszyn, Anne Baroin-Tourancheau, Jan Postberg, and Laurence Amar -- $t MicroRNA expression analysis using small RNA sequencing discovery and RT-qPCR-based validation / $r Alan Van Goethem, Pieter Mestdagh, Tom Van Maerken, and Jo Vandesompele -- $t Using FirePlex particle technology for multiplex microRNA profiling without RNA purification / $r Michael R. Tackett and Izzuddin Diwan -- $t Multiplex real-time PCR using encoded microparticles for microRNA profiling / $r Seungwon Jung and Sang Kyung Kim -- $t Optimized whole transcriptome profiling of motor axons / $r Lena Saal-Bauernschubert, Michael Briese, and Michael Sendtner -- $t 2D-DIGE in proteomics / $r Matias Pasquali, Tommaso Serchi, Sebastien Planchon, and Jenny Renaut -- $t STAGE-diging in proteomics / $r Paolo Soffientini and Angela Bachi -- $t Protein arrays I : antibody arrays / $r Yulin Yuan, Zuan-Tao Lin, Hongting Wang, Xia Hong, Mikala Heon, and Tianfu Wu -- $t Protein arrays II : antigen arrays / $r Yulin Yuan, Hongting Wang, Zuan-Tao Lin, Xia Hong, Mikala Heon, and Tianfu Wu -- $t Protein arrays III : reverse-phase protein arrays / $r Yulin Yuan, Xia Hong, Zuan-Tao Lin, Hongting Wang, Mikala Heon, and Tianfu Wu -- $t Isolation of exosomes for the purpose of protein cargo analysis with the use of mass spectrometry / $r Monika Pietrowska, Sonja Funk, Marta Gawin, Åukasz Marczak, Agata Abramowicz, Piotr WidÅak, and Theresa Whiteside -- $t Virus-induced gene silencing (VIGS) and foreign gene expression in Pisum sativum L. Using the "One-Step" Bean pod mottle virus (BPMV) viral vector / $r Chouaıb Meziadi, Sophie Blanchet, Valerie Geffroy, and Stephanie Pflieger -- $t Re-expressing epigenetically silenced genes by inducing DNA demethylation through targeting of ten-eleven translocation 2 to any given genomic locus / $r Julio Cesar Rendon, David Cano-Rodrıguez, and Marianne G. Rots -- $t Knockdown of rice microRNA166 by short tandem target mimic (STTM) / $r Sachin Teotia, Dabing Zhang, and Guiliang Tang -- $t RNAi-mediated knockdown of protein expression / $r Volker Baumann, Cornelia Lorenzer, Michael Thell, Anna-Maria Winkler, and Johannes Winkler -- $t Engineered zinc finger DNA-binding domains : synthesis, assessment of DNA-binding affinity, and direct protein delivery to mammalian cells / $r Mir A. Hossain, Isaac J. Knudson, Shaleen Thakur, Yong Shen, Jared R. Stees, Joeva J. Barrow, and Jorg Bungert -- $t Production, purification, and titration of first-generation adenovirus vectors / $r Ramona F. Kratzer and Florian Kreppel. 650 0 $a Functional genomics $v Laboratory manuals. 650 4 $a Microarreglos del aÌcido desoxirribonucleico $v Manuales de laboratorio. 650 4 $a GenoÌmica $v Manuales de laboratorio. 650 4 $a ProteoÌmica $v Manuales de laboratorio. 650 7 $a Functional genomics. $2 fast $0 (OCoLC)fst01753675 655 7 $a Laboratory manuals. $2 lcgft 655 7 $a Laboratory manuals. $2 fast $0 (OCoLC)fst01920776 700 1 $a Kaufmann, M. $q (Michael), $e editor. 700 1 $a Klinger, Claudia, $e editor. 700 1 $a Savelsbergh, Andreas, $e editor. 773 18 $w 990001282000202771 $g v:1654 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1654. $x 1064-3745 830 0 $a Springer protocols (Series), $x 1949-2448 941 $a 1 952 $l OVUX522 $d 20181116030318.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=9436D1F8580511E8A8F83C5097128E48Initiate Another SILO Locator Search