505-00 Clinical and laboratory diagnosis of peroxisomal disorders / Ronald J.A. Wanders, Femke C.C. Klouwer, Sacha Ferdinandusse, Hans R. Waterham, and Bwee Tien Poll-Thé. Isolation of peroxisomes from mouse brain using a continuous nycodenz gradient : a comparison to the isolation of liver and kidney peroxisomes / Miriam J. Schönenberger and Werner J. Kovacs -- Determining the topology of peroxisomal proteins using protease protection assays / Tânia Francisco, Ana F. Dias, Ana G. Pedrosa, Cláudia P. Grou, Tony A. Rodrigues, and Jorge E. Azevedo -- Isolation of native soluble and membrane-bound protein complexes from yeast Saccharomyces cerevisiae / Tobias Hansen, Anna Chan, Thomas Schröter, Daniel Schwerter, Wolfgang Girzalsky, and Ralf Erdmann -- Method for measurement of peroxisomal very long-chain fatty acid beta-oxidation and de novo C26:0 synthesis activity in living cells using stable-isotope labeled docosanoic acid / Malu-Clair van de Beek, Inge M.E. Dijkstra, and Stephan Kemp -- Analysis of plasmalogen synthesis in cultured cells / Masanori Honsho and Yukio Fujiki -- Transfection of primary human skin fibroblasts for peroxisomal studies / Janet Koster and Hans R. Waterham -- siRNA-mediated silencing of peroxisomal genes in mammalian cells / Tina A. Schrader and Michael Schrader -- Dual reporter systems for the analysis of translational readthrough in mammals / Julia Hofhuis, Severin Dieterle, Rosemol George, Fabian Schueren, and Sven Thoms -- Cytochemical detection of peroxisomes in light and electron microscopy with 3,3'-diaminobenzidine / H. Dariush Fahimi -- Cryo-immuno electron microscopy of peroxisomal marker proteins / Karina Mildner and Dagmar Zeuschner -- Detection and immunolabeling of peroxisomal proteins / Tina A. Schrader, Markus Islinger, and Michael Schrader -- Labeling of peroxisomes for live cell imaging in the filamentous fungus Ustilago maydis / Sofia C. Guimarães, Sreedhar Kilaru, Michael Schrader, and Martin Schuster -- Quantitative monitoring of subcellular redox dynamics in living mammalian cells using RoGFP2-based probes / Celien Lismont, Paul A. Walton, and Marc Fransen -- KillerRed as a tool to study the cellular responses to peroxisome-derived oxidative stress / Marc Fransen and Chantal Brees -- Determination of peroxisomal pH in living mammalian cells using pHRed / Luis F. Godinho and Michael Schrader -- In cellulo approaches to study peroxisomal protein import -- yeast immunofluorescence microscopy / Tobias Hansen, Wolfgang Girzalsky, and Ralf Erdmann -- Blue native PAGE : applications to study peroxisome biogenesis / Kanji Okumoto, Shigehiko Tamura, and Yukio Fujiki -- In vitro PMP import analysis using cell-free synthesized PMP and isolated peroxisomes / Yuqiong Liu, Masanori Honsho, and Yukio Fujiki -- Peroxisomal membrane and matrix protein import using a semi-intact mammalian cell system / Kanji Okumoto, Masanori Honsho, Yuqiong Liu, and Yukio Fujiki -- Use of glycosylation tags as reporters for protein entry into the endoplasmic reticulum in yeast and mammalian cells / Judith Buentzel and Sven Thoms -- Detection of ubiquitinated peroxisomal proteins in yeast / Natasha Danda and Chris Williams -- Assessing pexophagy in mammalian cells / Shun-ichi Yamashita and Yukio Fujiki -- Experimental systems to study yeast pexophagy / Shun-ichi Yamashita, Masahide Oku, Yasuyoshi Sakai, and Yukio Fujiki -- Flow cytometric analysis of the expression pattern of peroxisomal proteins, Abcd1, Abcd2, and Abcd3 in BV-2 murine microglial cells / Meryam Debbabi, Thomas Nury, Imen Helali, El Mostafa Karym, Flore Geillon, Catherine Gondcaille, Doriane Trompier, Amina Najid, Sébastien Terreau, Maryem Bezine, Amira Zarrouk, Anne Vejux, Pierre Andreoletti, Mustapha Cherkaoui-Malki, Stéphane Savary, and Gérard Lizard -- Study of peroxisomal protein phosphorylation by functional proteomics / Andreas Schummer, Sven Fischer, Silke Oeljeklaus, and Bettina Warscheid -- Analysis of peroxisomal β-oxidation during storage oil mobilization in Arabidopsis thaliana seedlings / Björn Hielscher, Lennart Charton, Tabea Mettler-Altmann, and Nicole Linka -- Peroxisome mini-libraries : systematic approaches to study peroxisomes made easy / Noa Dahan, Maya Schuldiner, and Einat Zalckvar -- Generation of peroxisome-deficient somatic animal cell mutants / Kanji Okumoto and Yukio Fujiki -- Clinical and laboratory diagnosis of peroxisomal disorders / Ronald J.A. Wanders, Femke C.C. Klouwer, Sacha Ferdinandusse, Hans R. Waterham, and Bwee Tien Poll-Thé.
Summary:
This volume provides easily accessible and comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Chapters detail valuable instructions, guidelines and protocols for molecular cell biologists, biochemists and biomedical researchers with an interest in peroxisome biology. Chapters in Peroxisomes: Methods and Protocols illustrate the isolation of peroxisomes, investigation of properties of membrane proteins, protocols to investigate and manipulate peroxisomes in cellular systems, detection of peroxisomes, including immunofluorescence, cytochemistry, cryo-immuno electron microscopy, and live cell imaging approaches. Authoritative and practical, Peroxisomes: Methods and Protocols aims to be useful for those already working on peroxisomes as well as for those who would like to start working on this fascinating organelle.
Series:
Methods in molecular biology, 1064-3745 ; 1595 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.