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Title:
DNA repair protocols.
Edition:
3rd ed. / edited by Lotte Bjergbæk.
Publisher:
Humana Press ;
Copyright Date:
c2012
Description:
xvii, 631 p. : ill. (some col.) ; 27 cm.
Subject:
DNA repair--Laboratory manuals.
DNA Repair--Laboratory Manuals.
Other Authors:
Bjergbæk, Lotte.
Notes:
Includes bibliographical references and index.
Contents:
Quantitative DNA damage and repair measurement with the yeast comet assay / Wiebke Schlörmann and Michael Glei -- Identification of genes required for damage survival using a cell-based RNAi screen against the Drosophila genome / Dashnamoorthy Ravi and Alexander James Roy Bishop -- Isolating genes involved with genotoxic drug response in the nematode Caenorhabditis elegans using genome-wide RNAi screening / Lone Vedel Schøler ... [et al.] -- Establishment of the DNA repair-defective mutants in DT40 cells / Masamichi Ishiai, Emi Uchida, and Minoru Takata -- Electrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair / Chun Tsai ... [et al.] -- Comet assay : a sensitive genotoxicity test for the detection of DNA damage and repair / Günter Speit and Andreas Rothfuss -- Detection of DNA damage by comet fluorescence in situ hybridization / Wiebke Schlörmann and Michael Glei -- Quantitative DNA damage and repair measurement with the yeast comet assay /
Milica Enoiu ... [et al.] -- Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts / Amy M. Furda ... [et al.] -- In vitro DNA mismatch repair in human cells / Liya Gu, Charles M. Ensor, and Guo-Min Li -- Eukaryotic DNA mismatch repair in vitro / Fenghua Yuan ... [et al.] -- Immuno-slot blot assay for detection of UVR-mediated DNA damage / Mahsa Karbaschi ... [et al.] -- Quantification of DNA photoproducts in mammalian cell DNA using radioimmunoassay / Thomas R. Berton and David L. Mitchell -- Measuring the formation and repair of UV damage at the DNA sequence level by ligation-mediated PCR / Ahmad Besaratinia and Gerd P. Pfeifer -- Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies / Milica Enoiu ... [et al.] -- Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts /
Eva-Maria Holstein and David Lydall -- Simple, non-radioactive measurement of single-stranded DNA at telomeric, sub-telomeric, and genomic loci in budding yeast / Jason L. Parsons and Grigory L. Dianov -- Using Arabidopsis cell extracts to monitor repair of DNA base damage in vitro / Dolores Córdoba-Cañero, Teresa Roldán-Arjona, and Rafael R. Ariza -- In vitro monitoring of base excision repair in Saccharomyces cerevisiae / Simone Moertl and Fred Ahne -- Mitochondrial base excision repair assays / Ricardo Gredilla and Tinna Stevnsner -- O-hydroxylamine-coupled alkaline gel electrophoresis assay for the detection and measurement of DNA single-strand breaks / April M. Luke and Jun Nakamura -- DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis / Helen E. Bryant -- Quantitative amplification of single-stranded DNA / Eva-Maria Holstein and David Lydall -- Simple, non-radioactive measurement of single-stranded DNA at telomeric, sub-telomeric, and genomic loci in budding yeast /
Jennifer J. Rahn, Gerald M. Adair, and Rodney S. Nairn -- In vitro rejoining of double strand breaks in genomic DNA / Neal Sugawara and James E. Haber -- Monitoring I-SceI-induced double-strand break repair in DT40 Cells / David du Plessis and Julian E. Sale -- I-SceI-based assays to examine distinct repair outcomes of mammalian chromosomal double strand breaks / Amanda Gunn and Jeremy M. Stark -- Studying repair of a single protein-bound nick in vivo using the Flp-nick system / Ida Nielsen, Anni H. Andersen, and Lotte Bjergbæk -- Measurement of diversification in the immunoglobulin light chain gene of DT40 cells / Julian E. Sale -- Live cell microscopy of DNA damage response in Saccharomyces cerevisiae / Sonia Silva ... [et al.] -- Use of gene targeting to study recombination in mammalian cell DNA repair mutants / Jennifer J. Rahn, Gerald M. Adair, and Rodney S. Nairn -- In vitro rejoining of double strand breaks in genomic DNA /
Arishya Sharma, Kamini Singh, and Alexandru Almasan. Histone H2AX phosphorylation : a marker for DNA damage / Kamal Datta ... [et al.] -- Assays of bypass replication of genotoxic lesions in cell-free extracts / Nana Nikolaishvili-Feinberg and Marila Cordeiro-Stone -- Quantitative measurement of translesion DNA synthesis in mammalian cells / Omer Ziv ... [et al.] -- Detection of PCNA modifications in Saccharomyces cerevisiae / Adelina A. Davies and Helle D. Ulrich -- In vitro PCNA modification assays / Joanne L. Parker and Helle D. Ulrich -- Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication / George Iliakis ... [et al.] -- CHK1 kinase activity assay / Hong Yan Wang and Ya Wang -- Histone H2AX phosphorylation : a marker for DNA damage / Arishya Sharma, Kamini Singh, and Alexandru Almasan.
Series:
Methods in molecular biology, 1064-3745 ; 920
Springer protocols
ISBN:
1617799971 (hbk. : alk. paper)
9781617799976 (hbk. : alk. paper)
OCLC:
(OCoLC)800860433
LCCN:
2012944173
Locations:
OVUX522 -- University of Iowa Libraries (Iowa City)

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