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05691aam a2200397 i 4500 001 8F37DC24580511E8A8F83C5097128E48 003 SILO 005 20180515010114 008 170610t20172017nyua b 001 0deng d 010 $a 2017950215 020 $a 1493972855 020 $a 9781493972852 035 $a (OCoLC)989966196 040 $a BTCTA $b eng $c BTCTA $d YDX $d STF $d OCLCO $d OCLCA $d OCLCF $d NUI $d SILO 245 00 $a Plant germline development : $b methods and protocols / $c edited by Anja Schmidt. 264 1 $a New York, NY : $b Humana Press ; $c [2017] 300 $a xiii, 422 pages : $b illustrations (some color) ; $c 26 cm 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v v. 1669 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographical references and index. 505 00 $t Conditional modulation of biological processes by low-temperature degrons / $r Nico Dissmeyer. $t Apomixis : engineering the ability to harness hybrid vigor in crop plants / $r Joann A. Conner and Peggy Ozias-Akins -- $t 3D imaging of whole-mount ovules at cellular resolution to study female germline development in rice / $r Ethel Mendocilla-Sato, Wenjing She, and Celia Baroux -- $t Live-cell imaging of F-actin dynamics during fertilization in Arabidopsis thaliana / $r Daichi Susaki, Daisuke Maruyama, Ramesh Yelagandula, Frederic Berger, and Tomokazu Kawashima -- $t Development and observation of mature megagametophyte cell-specific fluorescent markers / $r Mark A. Chamberlin and Shai J. Lawit -- $t Analysis of fluorescent reporter activity in the male germline during pollen development by confocal microscopy / $r David Twell and Lynette Brownfield -- $t In vivo ploidy determination of Arabidopsis thaliana male and female gametophytes / $r Burcu Nur Keceli, Nico De Storme, and Danny Geelen -- $t Staining and clearing of arabidopsis reproductive tissue for imaging of fluorescent proteins / $r Daniel Slane, Patrick Burgel, and Martin Bayer -- $t Live-cell imaging of auxin and cytokinin signaling in maize female gametophytes / $r Antony M. Chettoor and Matthew M.S. Evans -- $t Imaging Ca2+ dynamics in wild-type and NADPH oxidase-deficient mutant pollen tubes with yellow cameleon and confocal laser scanning microscopy / $r Christina Maria Franck, Jens Westermann, and Aurelien Boisson-Dernier -- $t Immunolocalization of AGPs and pectins in Quercus suber gametophytic structures / $r Mario Luıs da Costa, Ana Lucia Lopes, Maria Isabel Amorim, and Sılvia Coimbra -- $t Optimization of cell spreading and image quality for the study of chromosomes in plant tissues / $r Laksana Kantama, Erik Wijnker, and Hans de Jong -- $t Whole mount RNA-FISH on ovules and developing seeds / $r Andrea Bleckmann and Thomas Dresselhaus -- $t Analysis of peroxisome biogenesis in pollen by confocal microscopy and transmission electron microscopy / $r Peng-Fei Jia, Hong-Ju Li, and Wei-Cai Yang -- $t Transmission electron microscopy (TEM) to study histology of pollen and pollen tubes / $r Peng-Fei Jia, Hong-Ju Li, and Wei-Cai Yang -- $t Isolation of Arabidopsis pollen, sperm cells, and vegetative nuclei by fluorescence-activated cell sorting (FACS) / $r Mario R. Santos, Claudia Bispo, and Jorg D. Becker -- $t Isolation of rice sperm cells for transcriptional profiling / $r Scott D. Russell, Daniel S. Jones, Sarah Anderson, Xinkun Wang, Venkatesan Sundaresan, and Xiaoping Gou -- $t Manual isolation of living cells from the Arabidopsis thaliana female gametophyte by micromanipulation / $r Maria Englhart, Lucija Soljic, and Stefanie Sprunck -- $t Isolation and detection of small RNAs from pollen / $r German Martinez -- $t Analysis of proteins enriched in rice gamete / $r Takashi Okamoto -- $t Phosphoprotein enrichment from tobacco mature pollen crude protein extract / $r Jan Fıla and David Honys -- $t Identification of cis-regulatory modules that function in the male germline of flowering plants / $r Benjamin Peters, Jack Aidley, Murray Cadzow, David Twell, and Lynette Brownfield -- $t RNA-seq data analysis : from raw data quality control to differential expression analysis / $r Weihong Qi, Ralph Schlapbach, and Hubert Rehrauer -- $t RNA-seq data analysis protocol : combining in-house and publicly available data / $r Marc W. Schmid -- $t Impedance flow cytometry as a tool to analyze microspore and pollen quality / $r Iris Heidmann and Marco Di Berardino -- $t Agrobacterium-mediated Sorghum transformation / $r Emily Wu and Zuo-Yu Zhao -- $t Use of the Cas9 orthologs from Streptococcus thermophilus and Staphylococcus aureus for non-homologous end-joining mediated site-specific mutagenesis in Arabidopsis thaliana / $r Jeannette Steinert, Carla Schmidt, and Holger Puchta -- $t Transgenic reproductive cell ablation / $r Shai J. Lawit and Mark A. Chamberlin -- $t In vivo reporters for protein half-life / $r Pavel Reichman and Nico Dissmeyer -- $t Conditional modulation of biological processes by low-temperature degrons / $r Nico Dissmeyer. 650 0 $a Plants $x Development $v Laboratory manuals. 650 0 $a Plant molecular genetics $v Laboratory manuals. 650 2 $a Germ Cells, Plant. $0 (DNLM)D055993 650 2 $a Gametogenesis, Plant. $0 (DNLM)D056130 650 7 $a Plant molecular genetics. $2 fast $0 (OCoLC)fst01065562 650 7 $a Plants $x Development. $2 fast $0 (OCoLC)fst01065851 655 7 $a Laboratory manuals. $2 lcgft 655 7 $a Laboratory manuals. $2 fast $0 (OCoLC)fst01920776 700 1 $a Schmidt, Anja, $d 1980- $e editor. 773 18 $w 990001282000202771 $g v:1669 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1669, $x 1064-3745 830 0 $a Springer protocols (Series), $x 1949-2448 941 $a 1 952 $l OVUX522 $d 20181116050329.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=8F37DC24580511E8A8F83C5097128E48Initiate Another SILO Locator Search