Rhizosphere sampling protocols for microbiome (16S/18S/ITS rRNA) library preparation and enrichment for the isolation of drought tolerance-promoting microbes / Venkatachalam Lakshmanan, Prasun Ray, and Kelly D. Craven. Fundamental role of reactive oxygen species in plant stress response / Michael Liebthal and Karl-Josef Dietz -- Role of long noncoding RNAs in plant stress tolerance / Yuepeng Song and Deqiang Zhang -- Toward a resilient, functional microbiome : drought tolerance-alleviating microbes for sustainable agriculture / Venkatachalam Lakshmanan, Prasun Ray, and Kelly D. Craven -- Mining and quantifying in vivo molecular interactions in abiotic stress acclimation / Thorsten Seidel and Derya Kirasi -- Generation of a stress-inducible luminescent Arabidopsis and its use in genetic screening for stress-responsive gene deregulation mutants / Si-in Yu and Byeong-ha Lee -- Detection of differential DNA methylation under stress conditions using bisulfite sequence analysis / Ibtisam Al Harrasi, Rashid Al-Yahyai, and Mahmoud W. Yaish -- ChIP-seq analysis for identifying genome-wide histone modifications associated with stress-responsive genes in plants / Guosheng Li, Guru Jagadeeswaran, Andrew Mort, and Ramanjulu Sunkar -- Isolation of polysomal RNA for analyzing stress-responsive genes regulated at the translational level in plants / Yong-Fang Li, Ramamurthy Mahalingam, and Ramanjulu Sunkar -- Global proteomic profiling and identification of stress-responsive proteins using two-dimensional gel electrophoresis / Pragya Barua, Dipak Gayen, Nilesh Vikram Lande, Subhra Chakraborty, and Niranjan Chakraborty -- Phosphoproteomics analysis for probing plant stress tolerance / Christof Rampitsch -- Probing posttranslational redox modifications / Patrick Treffon, Michael Liebthal, Wilena Telman, and Karl-Josef Dietz -- Zymographic method for distinguishing different classes of superoxide dismutases in plants / Ashwini R. Jamdhade, Ramanjulu Sunkar, and Vandana K. Hivrale -- Determination of enzymes associated with sulfite toxicity in plants : kinetic assays for SO, APR, SiR, and In-Gel SiR activity / Galina Brychkova, Assylay Kurmanbayeva, Aizat Bekturova, Inna Khozin, Dominic Standing, Dmitry Yarmolinsky, and Moshe Sagi -- Determination of total sulfur, sulfate, sulfite, thiosulfate, and sulfolipids in plants / Assylay Kurmanbayeva, Galina Brychkova, Aizat Bekturova, Inna Khozin, Dominic Standing, Dmitry Yarmolinsky, and Moshe Sagi -- Determining glutathione levels in plants / Smita Sahoo, Jay Prakash Awasthi, Ramanjulu Sunkar, and Sanjib Kumar Panda -- Porous graphitic carbon liquid chromatography-mass spectrometry analysis of drought stress-responsive raffinose family oligosaccharides in plant tissues / Tiago F. Jorge, Maria H. Florêncio, and Carla António -- Profiling abscisic acid-induced changes in fatty acid composition in mosses / Suhas Shinde, Shivakumar Devaiah, and Aruna Kilaru -- Detection of free polyamines in plants subjected to abiotic stresses by high-performance liquid chromatography (HPLC) / Xiaoqing Gong and Ji-Hong Liu -- Determination of polyamines by dansylation, benzoylation, and capillary electrophoresis / Gounipalli Veeranagamallaiah and Chinta Sudhakar -- Rapid quantification of abscisic acid by GC-MS/MS for studies of abiotic stress response / Paul E. Verslues -- Silencing of stress-regulated miRNAs in plants by short tandem target mimic (STTM) approach / Sachin Teotia and Guiliang Tang -- Rhizosphere sampling protocols for microbiome (16S/18S/ITS rRNA) library preparation and enrichment for the isolation of drought tolerance-promoting microbes / Venkatachalam Lakshmanan, Prasun Ray, and Kelly D. Craven.
Summary:
This second edition provides up-to-date and new chapters covering the most important widely-used techniques in plant stress tolerance, including cutting-edge strategies, in a manner that ensures effective results. Plant Stress Tolerance: Methods and Protocols, Second Edition guides readers through reviews on epigenetics, long noncoding RNAs, and the influence of microbiome on drought tolerance, the volume then continues with methods describing the identification of stress-responsive genes, proteins, posttranslational redox modifications, DNA methylation, histone modifications, measurement of glutathione, lipids, raffinose family oligosaccharides, polyamines, as well as quantification of abscisic acid, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Stress Tolerance: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Series:
Methods in molecular biology, 1064-3745 ; 1631 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.