Single-cell dissociation and characterization in the murine retina and optic nerve / Hee Joo Choi, Rui Wang, and Tatjana C. Jakobs. Hypertonic saline injection model of experimental glaucoma in rats / John C. Morrison, Elaine C. Johnson, and William O. Cepurna -- Microbead occlusion model of ocular hypertension in mice / David J. Calkins, Wendi S. Lambert, Cathryn R. Formichella, William M. McLaughlin, and Rebecca M. Sappington -- Ocular hypertension/glaucoma in minipigs : episcleral veins cauterization and microbead occlusion methods / Elena Vecino, Haritz Urcola, Alejando Bayon, and Sansar C. Sharma -- Noninvasive intraocular pressure measurement in animals models of glaucoma / Yan Hu and John Danias -- High-throughput binocular pattern electroretinograms in the mouse / Tsung-Han Chou, Jonathon Toft-Nielsen, and Vittorio Porciatti -- Visual evoked potentials in glaucoma and alzheimer's disease / Elisa Cerri, Carlotta Fabiani, Chiara Criscuolo, and Luciano Domenici -- Investigation of the functional retinal output using microelectrode arrays / Gunther Zeck -- Quantitative proteomic analysis of human aqueous humor using iTRAQ 4plex labeling / Anna Trzeciecka, Padmanabhan Pattabiraman, Maria Carmen Piqueras, Carol Toris, and Sanjoy K. Bhattacharya -- Shotgun Sshingolipid analysis of human aqueous humor / Anna Trzeciecka, Ulises Arbelo, Arturo Barron, Genea Edwards, Sruthi Sampathkumar, Carol Toris, and Sanjoy K. Bhattacharya -- Assessment of aqueous humor dynamics in the rodent by constant flow infusion / J. Cameron Millar, Tien N. Phan, and Iok-Hou Pang -- Methods for analyzing endoplasmic reticulum stress in the trabecular meshwork of glaucoma models / Prabhavathi Maddineni, Ramesh B. Kasetti, and Gulab S. Zode -- Quantification of scleral biomechanics and collagen fiber alignment / Ian C. Campbell, Joseph M. Sherwood, Darryl R. Overby, Bailey G. Hannon, A. Thomas Read, Julia Raykin, and C. Ross Ethier -- Biolistic labeling of retinal ganglion cells / Luca Della Santina and Yvonne Ou -- Anterograde tract tracing for assaying axonopathy and transport deficits in glaucoma / Samuel D. Crish and Brett R. Schofield -- In vitro and in vivo methods for studying retinal ganglion cell survival and optic nerve regeneration / Yuqin Yin and Larry I. Benowitz -- 3D histomorphometric reconstruction and quantification of the optic nerve head connective tissues / Hongli Yang, Juan Reynaud, Howard Lockwood, Galen Williams, Christy Hardin, Luke Reyes, Stuart K. Gardiner, and Claude F. Burgoyne -- Visualizing astrocytes of the optic nerve / Daniel Sun -- Investigation of microRNA expression in experimental glaucoma / Hari Jayaram, Diana C. Lozano, Elaine C. Johnson, and John C. Morrison -- Utilizing RNA-seq to identify differentially expressed genes in glaucoma model tissues, such as the rodent optic nerve head / Diana C. Lozano, Dongseok Choi, Hari Jayaram, John C. Morrison, and Elaine C. Johnson -- Single-cell dissociation and characterization in the murine retina and optic nerve / Hee Joo Choi, Rui Wang, and Tatjana C. Jakobs.
Summary:
This detailed volume introduces the reader to current methods in glaucoma research, in particular those that make use of animal disease models. Given that the retinal ganglion cells damaged and destroyed by glaucoma do not regenerate after lowering the intraocular pressure, new therapeutic approaches that protect ganglion cells directly is a vital research goal that this volume?s contents aim to aid scientists in developing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Glaucoma: Methods and Protocols serves as a useful guide for both ophthalmologists in clinical practice and researchers in the field.
Series:
Methods in molecular biology, 1064-3745 ; 1695 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.