Constructing unmarked gene deletions in Legionella pneumophila / Carmen Buchrieser and Xavier Charpentier -- Identification of Legionella in clinical samples / Sophie Jarraud ... [et al.] -- Sequence-based identification of Legionella / Rodney M. Ratcliff -- Investigation of outbreaks : epidemiology / Carol Joseph -- Outbreak investigations and identification of Legionella in contaminated water / Susanne Lee and John Lee -- Typing methods for Legionella / Christian Lück ... [et al.] -- Culturing, media, and handling of Legionella / Christa H. Chatfield and Nicholas P. Cianciotto -- Intracellular metabolism of Legionella by isotopologue profiling / Klaus Heuner and Wolfgang Eisenreich -- Induction of competence for natural transformation in Legionella pneumophila and exploitation for mutant construction / Carmen Buchrieser and Xavier Charpentier -- Constructing unmarked gene deletions in Legionella pneumophila / Gary Faulkner and Rafael A. Garduño -- Pathogen vacuole purification from Legionella-infected amoeba and macrophages / Emilie Portier and Yann Hechard -- Production of L. pneumophila monospecies biofilms in a low-nutrient-concentration medium / Sophie Pécastaings and Christine Roques -- Enrichment of outer membrane vesicles shed by Legionella pneumophila / Jens Jäger and Michael Steinert -- Analysis of Legionella infection by flow cytometry / André N. Tiaden, Aline Kessler, and Hubert Hilbi -- Analysis of Legionella infection using RNAi in Drosophila cells / Dennise A. De Jesús, Tamara J. O'Connor, and Ralph R. Isberg -- Immunofluorescence imaging of Legionella infection in vitro / Stefan S. Weber and Annette Oxenius -- Electron microscopy of Legionella and Legionella-infected cells / Gary Faulkner and Rafael A. Garduño -- Pathogen vacuole purification from Legionella-infected amoeba and macrophages / Sharon G. Berk and Rafael A. Garduño -- Dictyostelium host response to Legionella infection : strategies and assays / Wenhan Zhu and Zhao-Qing Luo -- Subcellular localization of Legionella Dot/Icm effectors / Adam J. Vogrin ... [et al.] -- Purification and characterization of Legionella U-box-type E3 ubiquitin ligase / Hiroki Nagai and Tomoko Kubori -- Legionella phospholipases implicated in infection : determination of enzymatic activities / Kathleen Seipel and Antje Flieger -- Interactions of Legionella effector proteins with host phosphoinositide lipids / Stephen Weber, Stephanie Dolinsky, and Hubert Hilbi -- Characterization of Legionella lipopolysaccharide / Christian Lück and Jürgen H. Helbig -- Tetrahymena and acanthamoeba model system / Sharon G. Berk and Rafael A. Garduño -- Dictyostelium host response to Legionella infection : strategies and assays / Tobias Sahr and Carmen Buchrieser -- cDNA library construction for next-generation sequencing to determine the transcriptional landscape of Legionella pneumophila / Ann Karen C. Brassinga and Costi D. Sifri -- Infection of nonphagocytic host cells by Legionella / Shandra R. Day, Costi D. Sifri, and Paul S. Hoffman -- Analyzing caspase-1 activation during Legionella pneumophila infection in macrophages / Christopher L. Case and Craig R. Roy -- Mouse as a model for pulmonary Legionella infection / Juliana I. Hori and Dario S. Zamboni -- Assessment of Legionella-specific immunity in mice / Stefan S. Weber, Nicole Joller, and Annette Oxenius -- Guinea pig model of legionnaires' disease / Paul H. Edelstein -- Human susceptibility to legionnaires' disease / William R. Berrington and Thomas R. Hawn -- cDNA library construction for next-generation sequencing to determine the transcriptional landscape of Legionella pneumophila / Tobias Sahr and Carmen Buchrieser -- Identification of Legionella effectors using bioinformatic approaches / Gil Segal. Co-immunoprecipitation : protein-RNA and protein-DNA interaction / Tobias Sahr and Carmen Buchrieser -- Identification of Legionella effectors using bioinformatic approaches / Gil Segal.
Series:
Methods in molecular biology, 1064-3745 ; 954 Springer protocols
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