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Title:
Virus-host interactions : methods and protocols / edited by Susanne M. Bailer, Diana Lieber.
Publisher:
Humana Press ;
Copyright Date:
c2013
Description:
x, 372 p. : ill. (some col.) ; 27 cm.
Subject:
Virus Physiological Phenomena--Laboratory Manuals.
Host-Pathogen Interactions--Laboratory Manuals.
Virology--methods--Laboratory Manuals.
Viruses.
Host-virus relationships--Laboratory manuals.
Other Authors:
Bailer, Susanne M.
Lieber, Diana.
Notes:
Includes bibliographical references and index.
Contents:
Isolation and characterization of pathogen-bearing endosomes enable analysis of endosomal escape and identification of new cellular cofactors of infection / Lars Dölken -- Analysis of protein-protein interactions using LUMIER assays / Sonja Blasche and Manfred Koegl -- Detection of protein interactions during virus infection by bimolecular fluorescence complementation / Stefan Becker and Jens von Einem -- Discovery of host-viral protein complexes during Infection / Daniell L. Rowles, Scott S. Terhune, and Ileana M. Cristea -- Screening for host proteins with pro- and antiviral activity using high-throughput RNAi / Samantha J. Griffiths -- High resolution gene expression profiling of RNA synthesis, processing, and decay by metabolic labeling of newly transcribed RNA using 4-thiouridine / Lars Dölken -- Isolation and characterization of pathogen-bearing endosomes enable analysis of endosomal escape and identification of new cellular cofactors of infection /
Kerstin Laib Sampaio, Gerhard Jahn, and Christian Sinzger -- Applications for a dual fluorescent human cytomegalovirus in the analysis of viral entry / Caroline C. Friedel -- Interspecies heterokaryon assay to characterize the nucleocytoplasmic shuttling of herpesviral proteins / Shuai Wang, Kezheng Wang, and Chunfu Zheng -- Detection of integrated herpesvirus genomes by fluorescence in situ hybridization (FISH) / Benedikt B. Kaufer -- Fast generation of stable cell lines expressing fluorescent marker molecules to study pathogen induced processes / Jens Bernhard Bosse, Jessica Ragues, and Harald Wodrich -- Determination of HSV-1 infectivity by plaque assay and a luciferase reporter cell line / Diana Lieber and Susanne M. Bailer -- Generation of a stable cell line for constitutive miRNA expression / Diana Lieber -- Applications for a dual fluorescent human cytomegalovirus in the analysis of viral entry / Kerstin Laib Sampaio, Gerhard Jahn, and Christian Sinzger --
Assessment of natural killer cell responses to human cytomegalovirus-infected macrophages / Rebecca J. Aicheler and Richard J. Stanton -- Three-dimensional visualization of virus-infected cells by serial sectioning : an electron microscopic study using resin embedded cells / Martin Schauflinger, Clarissa Villinger, and Paul Walther -- 3D-tissue model for herpes simplex virus-1 infections / Ina Hogk, Steffen Rupp, and Anke Burger-Kentischer -- In vivo visualization of encephalitic lesions in herpes simplex virus type 1 (HSV-1) infected mice by magnetic resonance imaging (MRI) / Wali Hafezi and Verena Hoerr -- Detection of antigen-specific T cells based on intracellular cytokine staining using flow-cytometry / Tina Schmidt and Martina Sester -- Functional NK cell cytotoxicity assays against virus infected cells / Rebecca J. Aicheler and Richard J. Stanton -- Assessment of natural killer cell responses to human cytomegalovirus-infected macrophages /
Evgenia Scheibe ... [et al.]. Measurement of mouse cytomegalovirus-induced interferon-[beta] with immortalized luciferase reporter cells / Stefania Varani and Giada Frascaroli -- Gene targeting in mice : a review / Hicham Bouabe and Klaus Okkenhaug -- Protocol for construction of gene targeting vectors and generation of homologous recombinant embryonic stem cells / Hicham Bouabe and Klaus Okkenhaug -- Measurement of mouse cytomegalovirus-induced interferon-[beta] with immortalized luciferase reporter cells / Evgenia Scheibe ... [et al.].
Series:
Methods in molecular biology, 1064-3745 ; 1064
Springer protocols, 1949-2448
ISBN:
1627036016 (eBook)
9781627036016 (eBook)
9781627036009 (alk. paper)
1627036008 (alk. paper)
OCLC:
(OCoLC)843532433
LCCN:
2013944684
Locations:
OVUX522 -- University of Iowa Libraries (Iowa City)

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