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Title:
Glioblastoma : methods and protocols / edited by Dimitris G. Placantonakis.
Publisher:
Humana Press :
Copyright Date:
2018
Description:
xi, 241 pages : illustrations (some color) ; 26 cm.
Subject:
Gliomas--Laboratory manuals.
Gliomas.
Laboratory manuals.
Laboratory manuals.
Other Authors:
Placantonakis, Dimitris G., editor. http://id.loc.gov/authorities/names/no2018052634
Notes:
Includes bibliographical references and index.
Contents:
Modeling glioma with human embryonic stem cell-derived neural lineages / Aram S. Modrek, Jod Prado, Devin Bready, Joravar Dhaliwal, Danielle Golub, and Dimitris G. Placantonakis. Whole genome DNA methylation analysis of human glioblastoma using illumina BeadArrays / Jonathan Serrano and Matija Snuderl -- Establishing primary human glioblastoma adherent cultures from operative specimens / Amit D. Gujar, Diane D. Mao, John B. Finlay, and Albert H. Kim -- Establishing primary human glioblastoma tumorsphere cultures from operative specimens / Joshua D. Frenster and Dimitris G. Placantonakis -- Isolation of glioblastoma stem cells with flow cytometry / Julio Inocencio, Joshua D. Frenster, and Dimitris G. Placantonakis -- Lentiviral transduction of primary human glioblastoma cultures / Joshua D. Frenster, Julio Inocencio, and Dimitris G. Placantonakis -- Selective targeting of CD133-expressing glioblastoma stem cells using lentiviral vectors / N. Sumru Bayin and Dimitris G. Placantonakis -- Intracellular pH measurements in glioblastoma cells using the pH-sensitive dye BCECF / Nataliya V. Galifianakis, Dimitris G. Placantonakis, and Mitchell Chesler -- Induction and assessment of hypoxia in glioblastoma cells in vitro / Jean-Pierre Gagner, Mirna Lechpammer, and David Zagzag -- Metabolomic analysis of glioma cells using nanoflow liquid chromatography-tandem mass spectrometry / Jingjing Deng, Guoan Zhang, and Thomas A. Neubert -- Sample preparation for relative quantitation of proteins using tandem mass tags (TMT) and mass spectrometry (MS) / Hediye Erdjument-Bromage, Fang-Ke Huang, and Thomas A. Neubert -- Single-cell RNA sequencing of glioblastoma cells / Rajeev Sen, Igor Dolgalev, N. Sumru Bayin, Adriana Heguy, Aris Tsirigos, and Dimitris G. Placantonakis -- Evaluation of radioresponse and radiosensitizers in glioblastoma organotypic cultures / N. Sumru Bayin, Lin Ma, Dimitris G. Placantonakis, and Mary Helen Barcellos-Hoff -- Orthotopic patient-derived glioblastoma xenografts in mice / Zhongye Xu, Michael Kader, Rajeev Sen, and Dimitris G. Placantonakis -- Bioluminescent in vivo imaging of orthotopic glioblastoma xenografts in mice / Joshua D. Frenster and Dimitris G. Placantonakis -- Evaluation of vascularity, blood perfusion, and oxygen tension in tumor xenografts with fluorescent microscopy / N. Sumru Bayin and Dimitris G. Placantonakis -- Probing glioblastoma tissue heterogeneity with laser capture microdissection / Jean-Pierre Gagner and David Zagzag -- Flow cytometric identification of tumor-infiltrating lymphocytes from glioblastoma / Karolina Woroniecka, Pakawat Chongsathidkiet, Aladine Elsamadicy, Harrison Farber, Xiuyu Cui, and Peter E. Fecci -- Modeling glioma with human embryonic stem cell-derived neural lineages / Aram S. Modrek, Jod Prado, Devin Bready, Joravar Dhaliwal, Danielle Golub, and Dimitris G. Placantonakis.
Series:
Methods in molecular biology, 1064-3745 ; 1741
Springer protocols, 1949-2448
ISBN:
9781493976584
1493976583
OCLC:
(OCoLC)1007925127
LCCN:
2017964373
Locations:
OVUX522 -- University of Iowa Libraries (Iowa City)

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