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Title:
Phage display : a laboratory manual / by Carlos F. Barbas III ... [et al.].
Publisher:
Cold Spring Harbor Laboratory Press,
Copyright Date:
c2001
Description:
1 v. in various pagings : ill. ; 27 cm.
Subject:
Bacteriophages.--Laboratory manuals.
Microbial biotechnology.--Laboratory manuals.
Viral proteins.--Laboratory manuals.
Affinity chromatography.--Laboratory manuals.
Peptides--Biotechnology.--Laboratory manuals.
Proteins--Biotechnology.--Laboratory manuals.
Bacteriophages.--Laboratory manuals.
Antibodies, Monoclonal--analysis--Laboratory Manuals.
Chromatography, Affinity--methods--Laboratory Manuals.
Peptide Library--Laboratory Manuals.
Other Authors:
Barbas, Carlos F.
Notes:
Includes bibliographical references and index.
Contents:
Cell surface selection and analysis of monoclonal antibodies from phage libraries / D.L. Siegel. Phage-display vectors / J.K. Scott and C.F. Barbas III -- Antibody libraries / D.R. Burton -- Peptide libraries / J.K. Scott -- Functional domains and scaffolds / C.J. Silverman -- Gene fragment libraries and genomic and cDNA expression cloning / R.A. Williamson and G.J. Silverman -- Overview : Amplification and antibody genes / D.R. Burton -- Generation of antibody libraries : PCR amplification and assembly of light- and heavy-chain coding sequences / J. Andus-Widhopf...[et al.] -- Selection from antibody libraries / C. Rader, P. Steinberger, and C.F. Barbas III -- Analysis of selected antibodies / P. Steinberger, C. Rader, and C.F. Barbas III -- Production and purfification of Fab and scFv / M. Elia...[et al.] -- Antibody engineering / C. Rader and C.F. Barbas III -- Overview : Peptide libraries / J.K. Scott -- General phage methods / L.L.C. Bonnycastle...[et al.] -- Screening peptide libraries / A. Menendez...[et al.] -- Analysis of phage-borne peptides / M.B. Zwick...[et al.] -- Construction and use of pIII-displayed peptide libraries / K.A. Noren, L.H. Saltman, and C.J. Noren -- Construction and selection from gene fragment phage-display expression libraries / G.J. Silverman -- Construction and selection from cDNA phage-display expression libraries / G.J. Silverman -- In vivo selection of phage-display libraries / R. Pasqualini...[et al.] -- Cell surface selection and analysis of monoclonal antibodies from phage libraries / D.L. Siegel.
ISBN:
9780879695453 (pbk. : alk. paper)
0879695455 (pbk. : alk. paper)
9780879695460 (cloth : alk. paper)
0879695463 (cloth : alk. paper)
OCLC:
(OCoLC)43903550
LCCN:
00030834
Locations:
USUX851 -- Iowa State University - Parks Library (Ames)
OVUX522 -- University of Iowa Libraries (Iowa City)

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