Enhancing induced pluripotent stem cell generation by MicroRNA / Jason Dang and Tariq M. Rana -- Generation of human induced pluripotent stem cells from peripheral blood mononuclear cells using Sendai virus / Filipa A.C. Soares, Roger A. Pedersen, and Ludovic Vallier -- Sendai virus-based reprogramming of mesenchymal stromal/stem cells from umbilical cord wharton's jelly into induced pluripotent Stem Cells / Cristian Miere, Liani Devito, and Dusko Ilic -- Very rapid and efficient generation of induced pluripotent stem cells from mouse Pre-B cells / Bruno Di Stefano and Thomas Graf -- Generation of iPS cells from human peripheral blood mononuclear cells using episomal vectors / Ruijun Jeanna Su, Amanda Neises, and Xiao-Bing Zhang -- Enhancing induced pluripotent stem cell generation by MicroRNA / Jason Dang and Tariq M. Rana -- Computational biology methods for characterization of pluripotent cells / Anuja Mishra [and others] -- Using Oct4 : MerCreMer lineage tracing to monitor endogenous Oct4 expression during the reprogramming of fibroblasts into induced pluripotent stem cells (iPSCs) / Lucas V. Greder, Jason Post, and James R. Dutton -- Inducible transgene expression in human iPS cells using versatile all-in-one piggyBac transposons / Shin-Il Kim [and others] -- Generation and characterization of rat iPSCs / Jing Liao and Chun Cui -- Generation of induced pluripotent stem cells in rabbits / Marielle Afanassieff, Yann Tapponnier, and Pierre Savatier -- Cynomolgus monkey induced pluripotent stem cells generated by using allogeneic genes / Nobuhiro Shimozawa -- Induced pluripotent stem cells from nonhuman primates / Anuja Mishra [and others] -- Computational biology methods for characterization of pluripotent cells / Isıl Karanfil and Tugba Bagci-Onder -- Derivation of neural stem cells from mouse induced pluripotent stem cells / Ann M. Parr [and others] -- Analysis of the mitochondrial DNA and its replicative capacity in induced pluripotent stem cells / Gael Cagnone [and others] -- Selection of phage display peptides targeting human pluripotent stem cell-derived progenitor cell lines / Paola A. Bignone [and others] -- piggyBac transposon mediated reprogramming and flow cytometry analysis of CD44 and ICAM1 cell-surface marker changes / Sara Brightwell and Keisuke Kaji -- Generation and in vitro expansion of hepatic progenitor cells from human iPS cells / Ayaka Yanagida, Hiromitsu Nakauchi, and Akihide Kamiya -- Induced pluripotent stem (iPS) cell culture methods and induction of differentiation into endothelial cells / Ishita Chatterjee [and others] -- Derivation of neural stem cells from mouse induced pluripotent stem cells / Isıl Karanfil and Tugba Bagci-Onder -- Derivation of skeletal myogenic precursors from human pluripotent stem cells using conditional expression of PAX7 / Shiang Y. Lim [and others] -- Differentiation of iPSC to mesenchymal stem-like cells and their characterization / Kim Hynes [and others] -- Hepatic differentiation from human Ips cells using M15 cells / Kahoko Umeda, Nobuaki Shiraki, and Shoen Kume -- Determination of functional activity of human iPSC-derived hepatocytes by measurement of CYP metabolism / Pooja Chaudhari [and others] -- Induced pluripotent stem cells : generation, characterization, and differentiation-methods and protocols / Veronica Kon Graversen and Sai H. Chavala -- Mesoderm differentiation from hiPS cells / Hiroyuki Miwa and Takumi Era -- Enhancing human cardiomyocyte differentiation from induced pluripotent Stem Cells with Trichostatin A / Shiang Y. Lim [and others] -- Derivation of skeletal myogenic precursors from human pluripotent stem cells using conditional expression of PAX7 / Jian Mao and Lin Liu. Erratum to: Generation of iPS cells from granulosa cells / Ji-Yun Ko and Gun-Il Im -- Generation of iPS cells from granulosa cells / Jian Mao and Lin Liu -- Characteristics of murine iPS cells and siRNA transfection under hypoxia / K. Sugimoto and Yoshihiko Hayashi -- Hepatic differentiation from murine and human iPS cells using nanofiber scaffolds / Taiji Yamazoe, Nobuaki Shiraki, and Shoen Kume -- Erratum to: Generation of iPS cells from granulosa cells / Jian Mao and Lin Liu.
Summary:
This volume aims to be a collection of essential protocols in iPS cell field. Induced Pluripotent Stem (iPS) Cells: Methods and Protocols guides readers through multiple facets of stem cell biology, lineage commitment and differentiation. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols provides protocols that can be used by both experts and novices interested in stem cells.
Series:
Methods in molecular biology, 1064-3745 ; 1357 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.