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Title:
Allergy methods and protocols / edited by Meinir G. Jones and Penny Lympany.
Publisher:
Humana Press,
Copyright Date:
c2008
Description:
xi, 352 p. : ill.
Subject:
Allergy--Laboratory manuals.
Other Authors:
Jones, Meinir G.
Lympany, Penny.
Notes:
Includes bibliographical references and index.
Contents:
In situ hybridization / Kayhan T. Nouri-Aria. T cell : primary culture from peripheral blood / Monika Raulf-Heimsoth -- Production of T-cell lines / Helga Kahlert -- Production of human T-cell clones / Adrienne Verhoef -- Mapping of human T-cell epitopes of allergens / Thomas Zeiler and Tuomas Virtanen -- Determining MHC restriction of T-cell responses / Mark Larché -- Short-term culture of CD8 cells and intracellular cytokine staining / Beejal Vyas and Alistair Noble -- Isolation, flow cytometric analysis, and suppression assay of CD4+ CD25+ T-regulatory cells / Hayley Jeal -- Monocyte-derived dendritic cells as antigen-presenting cells in T-cell proliferation and cytokine production / Sun-sang J. Sung -- Ultrasensitive ELISA for measurement of human cytokine responses in primary culture / William P. Stefura ... [et al.] -- Quantification of human chemokine production in TLR-stimulated and antigen-specific recall responses / Monique Stinson ... [et al.] -- Standardization of allergen extracts / Jørgen Nedergaard Larsen and Sten Dreborg -- Immunoelectrophoresis for the characterization of allergen extracts / Gitte Nordskov Hansen and Jørgen Nedergaard Larsen -- Conjugation of haptens / Ranulfo Lemus and Meryl H. Karol -- Monoclonal antibodies / Helga Kahlert and Oliver Cromwell -- Purification of antibodies / Per H. Larsson -- Collection of air samples to quantify exposure to airborne allergens / Susan Gordon -- Assay of air sample eluates / Anne Renström and Susan Gordon -- The halogen assay : a new technique for measuring airborne allergen / Euan Tovey ... [et al.] -- Measurement of specific IgG anti-Fel d 1 antibodies / Meinir G. Jones -- The facilitated antigen binding (FAB) assay : a protocol to measure allergen-specific inhibitory antibody activity / James N. Francis -- Microscopic identification and purity determination of pollen grains / Magdalena Rahl -- Biopanning for the characterization of allergen mimotopes / Isabella Pali-Schöll and Erika Jensen-Jarolim -- Identification of mast cells and mast cell subpopulations / Mark Buckley and Andrew F. Walls -- Purification and characterization of mast cell tryptase and chymase from human tissues / Alan R. McEuen and Andrew F. Walls -- Experimental activation of mast cells and their pharmacological modulation / Shaoheng He and Andrew F. Walls -- In situ hybridization / Kayhan T. Nouri-Aria.
Series:
Methods in molecular medicine, 1543-1894 ; 138
ISBN:
1597453668 (e-ISBN)
9781597453660 (e-ISBN)
0896038963 (alk. paper)
9780896038967 (alk. paper)
LCCN:
2007932488
Locations:
USUX851 -- Iowa State University - Parks Library (Ames)

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