Screening for MAPK modulators using an In-Cell Western assay / Simon Schnaiter [and seven others] -- A novel method for the production of fully modified K-Ras 4B / Tanmay S. Chavan [and four others] -- Metabolic labeling of Ras with tritiated palmitate to monitor palmitoylation and depalmitoylation / Frederick D. Tsai [and three others] -- Ubiquitination of Rac1 by inhibitors of apoptosis (IAPs) / Tripat Kaur Oberoi-Khanuja and Krishnaraj Rajalingam -- Ras GTPases are both regulators and effectors of redox agents / Elisa Ferro [and four others] -- Biophysical and proteomic characterization strategies for cysteine modifications in Ras GTPases / G. Aaron Hobbs, Harsha P. Gunawardena, and Sharon L. Campbell -- Use of the yeast two-hybrid technology to isolate molecular interactions of Ras GTPases / Elisa Ferro, Eva Baldini, and Lorenza Trabalzini -- Screening for MAPK modulators using an In-Cell Western assay / Simon Schnaiter [and seven others] -- An in vitro system to evaluate the scaffold function of the RalA effector protein RalBP1 / David F. Kashatus -- Functional phosphoproteomics of oncogenic KRAS signaling / Putty-Reddy Sudhir and Jeou-Yuan Chen -- Pull-down assay for analysis of integrin-mediated activation of Rap proteins in adherent platelets / Gianni Francesco Guidetti and Mauro Torti -- Combined pulldown and time-lapse microscopy studies for determining the role of Rap1 in the crosstalk between integrins and cadherins / Luca Goitre and Saverio Francesco Retta -- Fluorescence microscopy study of Rap1 subcellular localization / Luca Goitre, Valentina Cutano, and Saverio Francesco Retta -- An in vitro system to evaluate the scaffold function of the RalA effector protein RalBP1 / David F. Kashatus -- Nanoclustering and heterogeneous membrane diffusion of Ras studied by FRAP and RICS analysis / Camilo Guzmán, Maja Šolman, and Daniel Abankwa -- Immunofluorescence methods in studies of the GTPase Ran and its effectors in interphase and in mitotic cells / Giulia Guarguaglini, Valeria de Turris, and Patrizia Lavia -- High-resolution scanning electron microscopy for the imaging of nuclear pore complexes and Ran-mediated transport / Lihi Shaulov, Boris Fichtman, and Amnon Harel -- Effector recruitment method to study spatially regulated activation of Ras and Rho GTPases / Lauren P. Huff, Molly J. DeCristo, and Adrienne D. Cox -- Real-time visualization and quantification of native Ras activation in single living cells / Christoph Biskup and Ignacio Rubio -- Nanoclustering and heterogeneous membrane diffusion of Ras studied by FRAP and RICS analysis / Camilo Guzmán, Maja Šolman, and Daniel Abankwa -- Ras proteins signaling in the early metazoan Dictyostelium discoideum / Enrico Bracco and Barbara Pergolizzi. Analysis of Rho GTPase-induced localization of nanoscale adhesions using fluorescence nanoscopy / Annica K.B. Gad [and three others] -- Yeast as a model for Ras signalling / Renata Tisi, Fiorella Belotti, and Enzo Martegani -- Methods to study the Ras2 protein activation state and the subcellular localization of Ras-GTP in Saccharomyces cerevisiae / Sonia Colombo and Enzo Martegani -- Ras proteins signaling in the early metazoan Dictyostelium discoideum / Enrico Bracco and Barbara Pergolizzi.
Series:
Methods in molecular biology, 1064-3745 ; 1120 Springer protocols, 1949-2448
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