Effectoromics-based identification of cell surface receptors in potato / Emmanouil Domazakis, Xiao Lin, Carolina Aguilera-Galvez, Doret Wouters, Gerard Bijsterbosch, Pieter J. Wolters, and Vivianne G.A.A. Vleeshouwers. Characterization of plant cell wall damage-associated molecular patterns regulating immune responses / Laura Bacete, Hugo Mélida, Sivakumar Pattathil, Michael G. Hahn, Antonio Molina, and Eva Miedes -- Methods of isolation and characterization of oligogalacturonide elicitors / Manuel Benedetti, Benedetta Mattei, Daniela Pontiggia, Gianni Salvi, Daniel Valentin Savatin, and Simone Ferrari -- Quantitative analysis of ligand-induced endocytosis of FLAGELLIN-SENSING 2 using automated image segmentation / Michelle E. Leslie and Antje Heese -- Analysis for protein glycosylation of pattern recognition receptors in plants / Takaakira Inokuchi and Yusuke Saijo -- Assays to investigate the N-glycosylation state and function of plant pattern recognition receptors / Stacey A. Lawrence, Teresa Ceserani, and Nicole K. Clay -- Steady-state and kinetics-based affinity determination in effector-effector target interactions / André Reinhard and Thorsten Nürnberger -- In vitro ubiquitination activity assays in plant immune responses / Giulia Furlan and Marco Trujillo -- Bioinformatics analysis of the receptor-like kinase (RLK) superfamily / Otávio J.B. Brustolini, José Cleydson F. Silva, Tetsu Sakamoto, and Elizabeth P.B. Fontes -- Identification of MAPK substrates using quantitative phosphoproteomics / Tong Zhang, Jacqueline D. Schneider, Ning Zhu, and Sixue Chen -- Analysis of PAMP-triggered ROS burst in plant immunity / Yuying Sang and Alberto P. Macho -- MAPK assays in Arabidopsis MAMP-PRR signal transduction / Hoo Sun Chung and Jen Sheen -- LeEIX2 interactors' analysis and EIX-mediated responses measurement / Meirav Leibman-Markus, Silvia Schuster, and Adi Avni -- CDPK activation in PRR signaling / Heike Seybold, Marie Boudsocq, and Tina Romeis -- Chitin and stress induced protein kinase activation / Chandra Kenchappa, Raquel Azevedo da Silva, Simon Bressendorff, Sabrina Stanimirovic, Jakob Olsen, Morten Petersen, and John Mundy -- Measuring callose deposition, an indicator of cell wall reinforcement, during bacterial infection in arabidopsis / Lin Jin and David M. Mackey -- Quantitative evaluation of plant actin cytoskeletal organization during immune signaling / Yi-Ju Lu and Brad Day -- Network Reconstitution for Quantitative Subnetwork Interaction Analysis / Fumiaki Katagiri -- Stomatal bioassay to characterize bacterial-stimulated PTI at the pre-invasion phase of infection / Jeanine Montano and Maeli Melotto -- Using clear nail polish to make Arabidopsis epidermal impressions for measuring the change of stomatal aperture size in immune response / Shuchi Wu and Bingyu Zhao -- Characterizing the immune-eliciting activity of putative microbe-associated molecular patterns in tomato / Christopher R. Clarke and Boris A. Vinatzer -- Genome-wide analysis of chromatin accessibility in arabidopsis infected with Pseudomonas syringae / Yogendra Bordiya and Hong-Gu Kang -- Small RNA and mRNA profiling of arabidopsis in response to Phytophthora infection and PAMP treatment / Yingnan Hou and Wenbo Ma -- Mapping and cloning of chemical induced mutations by whole-genome sequencing of bulked segregants / Jian Hua, Shuai Wang, and Qi Sun -- Rapid construction of multiplexed CRISPR-Cas9 systems for plant genome editing / Levi Lowder, Aimee Malzahn, and Yiping Qi -- Chitin-triggered MAPK activation and ROS generation in rice suspension-cultured cells / Koji Yamaguchi and Tsutomu Kawasaki -- Chitin-induced responses in the moss Physcomitrella patens / Simon Bressendorff, Magnus Wohlfahrt Rasmussen, Morten Petersen, and John Mundy -- Methods to quantify PAMP-triggered oxidative burst, MAP kinase phosphorylation, gene expression, and lignification in brassicas / Simon R. Lloyd, Christopher J. Ridout, and Henk-jan Schoonbeek -- Effectoromics-based identification of cell surface receptors in potato / Emmanouil Domazakis, Xiao Lin, Carolina Aguilera-Galvez, Doret Wouters, Gerard Bijsterbosch, Pieter J. Wolters, and Vivianne G.A.A. Vleeshouwers.
Summary:
This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Pattern Recognition Receptors: Methods and Protocolsaims to ensure successful results in the further study of this vital field.
Series:
Methods in molecular biology, 1064-3745 ; 1578 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.