Published In: United States, 10 December 2016"--Distributor's website." Includes bibliographical references and index.
Contents:
Monosynaptic tracing in developing circuits using modified rabies virus / Laura Cocas and Gloria Fernandez. Co-culture synaptogenic assay : a new look at fluorescence reporters and technological devices / Karen Perez de Arce and Massimiliano Stagi -- Synaptogenic assays using neurons cultured on micropatterned substrates / Katalin Czöndör and Olivier Thoumine -- Monitoring synapses via trans-synaptic GFP complementation / Theodoros Tsetsenis -- Generation of spinal motor neurons from human pluripotent stem cells / David P. Santos and Evangelos Kiskinis -- Biochemical purification of binding partners of synaptic scaffold proteins / Bekir Altas, Olaf Jahn, and Hiroshi Kawabe -- In situ protein binding assay using Fc-fusion proteins / Nirmala Padmanabhan and Tabrez J. Siddiqui -- Reconstitution of synaptic SNAREs into large liposomes with reduced curvature stress / Javier M. Hernandez -- Isolation of synaptosomes, synaptic plasma membranes, and synaptic junctional complexes / Mary L. Michaelis, Lei Jiang, and Elias K. Michaelis -- Purification of synaptosome populations using fluorescence-activated synaptosome sorting / Elisa Luquet, Christoph Biesemann, Annie Munier, and Etienne Herzog -- Optimized protocol for imaging cleared neural tissues using light microscopy / Yoh Isogai, Douglas S. Richardson, Catherine Dulac, and Joseph Bergan -- Structured illumination microscopy for the investigation of synaptic structure and function / Soyon Hong, Daniel K. Wilton, Beth Stevens, and Douglas S. Richardson -- 3D d STORM imaging of fixed brain tissue / Frank Herrmannsdörfer, Benjamin Flottmann, Siddarth Nanguneri, Varun Venkataramani, Heinz Horstmann, Thomas Kuner, and Mike Heilemann -- Photomarking relocalization technique for correlated two-photon and electron microcopy imaging of single stimulated synapses / Miquel Bosch, Jorge Castro, Mriganka Sur, and Yasunori Hayashi -- 3D analysis of synaptic ultrastructure in organotypic hippocampal slice culture by high-pressure freezing and electron tomography / Cordelia Imig and Benjamin H. Cooper -- Analyzing endosomal docking, fusion, sorting, and budding mechanisms in isolated organelles / Sina V. Barysch and Ioanna Bethani -- Concurrent imaging of receptor trafficking and calcium dynamics by spinning disk confocal microscopy / DeLaine D. Larsen, Regina Wai-Yan Choy, and Minjong Park -- Imaging activity-dependent signaling dynamics at the neuronal synapse using FRET-based biosensors / Zohreh Farsi and Andrew Woehler -- Analyzing structural plasticity of dendritic spines in organotypic slice culture / Mathias De Roo and Adema Ribic -- Using fluorescent markers to estimate synaptic connectivity in situ / Mrinalini Hoon, Raunak Sinha, and Haruhisa Okawa -- Dual anterograde and retrograde viral tracing of reciprocal connectivity / Matthias G. Haberl, Melanie Ginger, and Andreas Frick -- Mapping synaptic inputs of developing neurons using calcium imaging / Johan Winnubst and Christian Lohmann -- Monosynaptic tracing in developing circuits using modified rabies virus / Laura Cocas and Gloria Fernandez.
Series:
Methods in molecular biology, 1064-3745 ; 1538 Spencer protocols, 1949-2448
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