Methodology -- Basic methodology -- Manipulation of DNA by PCR / Kenshi Hayashi -- Cloning PCR products / Michael A. Frohman -- Optimization of multiplex PCRs / Jeffrey S. Chamberlain and Joel R. Chamberlain -- Preparation of nucleic acids for archival material / Darryl Shibata -- PCR amplification of viral DNA and viral host cell mRNAs in situ / Janet Embretson ... [et al.] -- Quantitation -- Quantitative PCR: an overview / F. Ferre ... [et al.] -- Quantification of DNAs by the polymerase chain reaction using an internal control / Donald M. Coen -- RT-PCR and mRNA quantitation / Jamel Chelly and Axel Kahn -- Analysis of human T-cell repertoires by PCR / Dominic G. Spinella and James M. Robertson -- Nonisotopic detection -- Ultrasensitive nonradioactive detection of PCR reactions: an overview / Richard H. Tullis -- Fluorescent detection methods for PCR analysis / Michael J. Heller -- Enzyme-labeled oligonucleotides / Eugene Tu and Edward Jablonski -- Application of the hybridization protection assay (HPA) to PCR / Norman C. Nelson and Sherrol H. McDonough. Instrumentation -- PCR instrumentation: where do we stand? / Christian C. Oste -- Rapid cycle DNA amplification / Carl T. Wittwer, Gudrun B. Reed and Kirk M. Ririe -- Automating the PCR process / H.R. Garner -- Sequencing -- PCR and DNA sequencing / Bjorn Andersson and Richard A. Gibbs -- Phage promoter-based methods for sequencing and screening for mutations / Steve S. Sommer and Erica L. Vielhaber -- Capture PCR: an efficient method for walking along chromosomal DNA and cDNA / Andre Rosenthal, Matthias Platzer and D. Stephen Charnock-Jones -- Applications -- General applications -- In vitro evolution of functional nucleic acids: high-affinity RNA ligands of the HIV-1 rev protein / Craig Tuerk ... [et al.] -- Application of PCR to forensic science / Bruce Budowle ... [et al.] -- Recreating the past by PCR / Matthias Hoss, Oliva Handt and Svante Paabo -- Nonbiological applications / Gavin Dollinger -- Genetic analysis -- RT-PCR and gene expression / Didier Montarras ... [et al.]. Fingerprinting using arbitrarily primed PCR: application to genetic mapping, population biology, epidemiology and detection of differentially expressed RNAs / John Welsh and Michael McClelland -- Genetics, plants and the polymerase chain reaction / Bruno W.S. Sobral and Rhonda J. Honeycutt -- Assessment of therapy effectiveness -- PCR assessment of the efficacy of therapy in Philadelphia chromosome-positive leukemias / Stephen P. Hunger and Michael L. Cleary -- Detection of minimal residual disease (MRD) in acute lymphoblastic leukemia using clone-specific probes directed against V(D)J junctional sequences / Luc d'Auriol and Francois Sigaux -- Assessment of therapy effectiveness: infectious disease / Salvatore J. Arrigon -- Gene therapy / Richard A. Morgan and W. French Anderson -- Diagnostics -- PCR and cancer diagnostics: detection and characterization of single point mutations in oncogenes and antioncogenes / Manuel Perucho -- Clinical applications of the polymerase chain reaction / Belinda J.F. Rossiter and C. Thomas Caskey. Infectious diseases / W. John Martin -- PCR and the world of business -- PCR in the marketplace / Ellen Daniell -- PCR and scientific invention: the trial of DuPont vs. Cetus / Kary B. Mullis.
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.