Isolation and characterization of mouse intrahepatic lymphocytes by flow cytometry / Florian Wiede and Tony Tiganis. Combining cell type-restricted adenoviral targeting with immunostaining and flow cytometry to identify cells-of-origin of lung cancer / Sarah A. Best, Ariena Kersbergen, Marie-Liesse Asselin-Labat, and Kate D. Sutherland -- Utility of endoscopic ultrasound-guided fine-needle aspiration for preclinical evaluation of therapies in cancer / William Berry and Daniel Croagh -- Modelling intestinal carcinogenesis using in vitro organoid cultures / Thierry Jarde, Genevieve Kerr, Reyhan Akhtar, and Helen E. Abud -- Protocols to evaluate cigarette smoke-induced lung inflammation and pathology in mice / Ross Vlahos and Steven Bozinovski -- Tracking competent host defence to chronic inflammation : an in vivo model of peritonitis / Javier Uceda Fernandez, David Millrine, and Simon A. Jones -- Citrobacter rodentium infection model for the analysis of bacterial pathogenesis and mucosal immunology / Catherine L. Kennedy and Elizabeth L. Hartland -- In vivo infection model of severe influenza A virus / Ashley Mansell and Michelle D. Tate -- In vivo models for inflammatory arthritis / Gareth W. Jones, David G. Hill, Katie Sime, and Anwen S. Williams -- Isolation of mouse primary gastric epithelial cells to investigate the mechanisms of Helicobacter pylori associated disease / Le Son Tran and Richard L. Ferrero -- Dissecting interleukin-6 classic- and trans-signaling in inflammation and cancer / Christoph Garbers and Stefan Rose-John -- Laser microdissection of cellular compartments for expression analyses in cancer models / Tae-Su Han and Masanobu Oshima -- Tissue processing for stereological analyses of lung structure in chronic obstructive pulmonary disease / Mohamed Saad and Saleela M. Ruwanpura -- Quantifying caspase-1 activity in murine macrophages / Dave Boucher, Amy Chan, Connie Ross, and Kate Schroder -- Analysis of histone modifications in acute myeloid leukaemia using chromatin immunoprecipitation / Benjamin J. Shields, Andrew Keniry, Marnie E. Blewitt, and Matthew P. McCormack -- Analysis of DNA methylation in tissues exposed to inflammation / Naoko Hattori and Toshikazu Ushijima -- Comprehensive protocol resource for performing pooled shRNA and CRISPR screens / Leonie A. Cluse, Iva Nikolic, Deborah Knight, Piyush B. Madhamshettiwar, Jennii Luu, Karla J. Cowley, Timothy Semple, Gisela Mir Arnau, Jake Shortt, Ricky W. Johnstone, and Kaylene J. Simpson -- Immuno-detection of immature and bioactive forms of the inflammatory cytokine IL-18 / Brendan J. Jenkins and Virginie Deswaerte -- Optimization techniques for miRNA expression in low frequency immune cell populations / Victoria G. Lyons, Natalie L. Payne, and Claire E. McCoy -- Assessing the cGAS-cGAMP-STING activity of cancer cells / Genevie`ve Pepin and Michael P. Gantier -- Expression and purification of JAK1 and SOCS1 for structural and biochemical studies / Nicholas P.D. Liau and Jeffrey J. Babon -- Production of recombinant killer immunoglobulin-like receptors for crystallography and luminex-based assays / Phillip Pymm and Julian P. Vivian -- Isolation and characterization of mouse intrahepatic lymphocytes by flow cytometry / Florian Wiede and Tony Tiganis.
Summary:
This volume provides a broad spectrum of research models, techniques, and protocols. Chapters are divided into sections providing detailed information on the background and context for the chosen topic of interest, a list of the necessary materials and reagents needed for each topic, as well as the step-by-step laboratory protocols and methodologies for the successful and reproducible execution of each topic. In addition, each chapter includes notes to provide tips avoiding known pitfalls, troubleshooting advice and elaborate further on specific materials or methods. uthoritative and practical, Inflammation and Cancer: Methods and Protocols aims to provide information to students and researchers at all levels of experience for further study into this vital field.
Series:
Springer protocols, 1949-2448 Methods in molecular biology, 1064-3745 ; v. 1725
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.