"Extras online"--Cover. Includes bibliographical references and index.
Contents:
Introduction / Yasunori Sasakura -- Microinjection of exogenous nucleic acids into eggs: Ciona species / Kenji Kobayashi, Yutaka Satou -- Practical guide for ascidian microinjection: Phallusia mammillata / Hitoyoshi Yasuo, Alex McDougall -- Microinjection of exogenous DNA into eggs of Halocynthia roretzi / Gaku Kumano -- Electroporation in ascidians: history, theory and protocols / Robert W. Zeller -- The use of cis-regulatory DNAs as molecular tools / Kotaro Shimai, Takehiro G. Kusakabe -- Reporter analyses reveal redundant enhancers that confer robustness on cis-regulatory mechanisms / Shigeki Fujiwara, Cristian CanĚestro -- Investigating evolutionarily conserved molecular mechanisms controlling gene expression in the notochord / Julie E. Maguire, Aakarsha Pandey, Yushi Wu, Anna Di Gregorio -- Purification of fluorescent labeled cells from dissociated Ciona embryos / Wei Wang, Claudia Racioppi, Basile Gravez, Lionel Christiaen -- Germline transgenesis in Ciona / Yasunori Sasakura -- The enhancer trap in Ciona / Yasunori Sasakura -- TALEN-based knockout system / Keita Yoshida, Nicholas Treen -- CRISPR knockouts in Ciona embryos / Shashank Gandhi, Florian Razy-Krajka, Lionel Christiaen, Alberto Stolfi -- Transgenic techniques for investigating cell biology during development / Christina D. Cota -- Cellular processes of notochord formation / William C. Smith -- Morphology and physiology of the ascidian nervous systems and the effectors / Atsuo Nishino.
Series:
Advances in experimental medicine and biology, 0065-2598 ; volume 1029
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