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Title:

Transgenesis techniques : principles and protocols / edited by Elizabeth J. Cartwright.

Edition:

3rd ed.

Publisher:

Humana Press,

Copyright Date:

c2009

Description:

xii, 335 p. : ill. (some col.) ; 27 cm.

Subject:

Transgenic animals--Laboratory manuals.
Animal genetic engineering--Laboratory manuals.

Other Authors:

Cartwright, Elizabeth J.

Notes:

Earlier edition published 2002. Includes bibliographical references and index.

Contents:

Transgenesis in Drosophila melanogaster / L. Ringrose -- Transgenesis in Caenorhabditis elegans / M. Rieckher ... [et al.] -- Transgenesis in zebrafish with the tol2 transposon system / M.L. Suster ... [et al.] -- Generation of transgenic frogs / J. Loeber, F.C. Pan and T. Pieler -- Pronuclear DNA injection for the production of transgenic rats / J. Cozzi ... [et al.] -- Cell-type-specific transgenesis in the mouse / J. Gulick and J. Robbins -- Transgene design and delivery into the mouse genome: keys to success / L. Teboul -- Overexpression transgenesis in mouse: pronuclear injection / W.J. Gardiner and L. Teboul -- Gene-targeting vectors / J. Simon, C. Arthur and V.A. McGuire -- Gene trap: knockout on the fast lane / M. Ullrich and K. Schuh -- Culture of murine embryonic stem cells / I. Barbaric and T.N. Dear -- Targeting embryonic stem cells / R.H. Friedel -- Generation of chimeras by microinjection / A. Pluck and C. Klasen -- Generation of chimeras by morula aggregation / A. Pluck and C. Klasen -- Surgical techniques for the generation of mutant mice / A. Pluck and C. Klasen -- Site-specific recombinases for manipulation of the mouse genome / M.C. Birling, F. Gofflot and X. Warot -- Cre transgenic mouse lines / X. Wang -- Large-scale mouse mutagenesis / E.J. Cartwright -- Dedicated mouse production and husbandry / L. Vizor and S. Wells -- Biological methods for archiving and maintaining mutant laboratory mice. Part I: conserving mutant strains / M.D. Fray -- Biological methods for archiving and maintaining mutant laboratory mice. Part II: recovery and distribution of conserved mutant strains / M.D. Fray.

Series:

Methods in molecular biology, 1064-3745 ; v.561
Springer protocols

ISBN:

1603270183 (alk. paper)
9781603270182 (alk. paper)

OCLC:

(OCoLC)432240562

LCCN:

2009929336

Locations:

USUX851 -- Iowa State University - Parks Library (Ames)

OVUX522 -- University of Iowa Libraries (Iowa City)