Detection of the Bacterial Potato Pathogens Pectobacterium and Dickeya spp. using conventional and real-time PCR / Humphris, Sonia N. (and others) -- Detection and Identification of Phoma Pathogens of Potato / A'Hara, Denise -- Diagnosis of Ramularia collo-cygni and Rhynchosporium spp. in Barley / Havis, Neil (and others) -- A Real-Time Multiplex PCR Assay Used in the Identification of Closely Related Fungal Pathogens at the Species Level / Wright, Dominie G. -- Diagnostics of Tree Diseases Caused by Phythophthora austrocedri Species / Mulholland, Vincent (and others) -- Real-Time LAMP for Chalara fraxinea Diagnosis / Tomlinson, Jenny (and others) -- Loop-Mediated Isothermal Amplification Procedure (LAMP) for Detection of the Potato Zebra Chip Pathogen / Ravindran, Aravind (and others) -- Loop-Mediated Isothermal Amplification (LAMP) for Detection of Phytoplasmas in the Field / Dickinson, Matt -- Diagnosis of Phytoplasmas by Real-Time PCR Using Locked Nucleic Acid (LNA) Probes / Palmano, Sabrina (and others) -- Q-Bank Phytoplasma: A DNA Barcoding Tool for Phytoplasma Identification / Contaldo, Nicoletta (and others) -- High-Throughput Diagnosis of Potato Cyst Nematodes in Soil Samples / Reid, Alex (and others) -- Detection of Nepovirus Vector and Nonvector Xiphenima Species in Grapevine / Ghelder, C. (and others) -- Molecular and Serological Methods for the Diagnosis of Viruses in Potato Tubers / Lacomme, Christophe (and others) -- Immunocapture-Multiplex RT-PCR for the Simultaneous Detection and Identification of Plant Viruses and Their Strains: Study Case, Potato Virus Y (PVY) / Chikh-Ali, Mohamad (and others) -- SNaPshot and CE-SSCP: Two Simple and Cost-Effective Methods to Reveal Genetic Variability Within a Virus Species / Delaunay, Agnès (and others) -- Detection and Characterization of Viral Species/Subspecies Using Isothermal Recombinase Polymerase Amplification (RPA) Assays / Glais, Laurent (and others) -- Virus Testing by PCR and RT-PCR Amplification in Berry Fruit / MacFarlane, Stuart (and others) -- Metagenomics Approaches Based on Virion-Associated Nucleic Acids (VANA): An Innovative Tool for Assessing Without A Priori Viral Diversity of Plants / Filloux, Denis (and others) -- Detection of Potato spindle tuber viroid and other related viroids by a DIB Labelled RNA Probe / Monger, Wendy A. (and others) -- Microarray Platform for the Detection of a Range of Plant Viruses and Viroids / Adams, Ian (and others) -- Multiplex Detection of Plant Pathogens Through the Luminex Magplex Bead System / Vlugt, René A. A. (and others) -- Next-Generation Sequencing of Elite Berry Germplasm and Data Analysis Using a Bioinformatics Pipeline for Virus Detection and Discovery / Ho, Thien (and others) -- Metagenomic Next-Generation Sequencing of Viruses Infecting Grapevines / Burger, Johan T. (and others) -- Droplet Digital PCR for Absolute Quantification of Pathogens / Gutiérrez-Aguirre, Ion (and others).
Summary:
"The second edition of Plant Pathology: Techniques and Protocols covers diagnostic methods that are currently used in laboratories for a broad range of plant species and matrixes. These include serological and molecular methods that have one or more of the following characteristics: suitability for high-throughput testing, detection of a group of pathogens or of sometimes uncharacterized pathogens, detection and identification of specific pathogens, and high sensitivity. This volume discusses qualitative and quantitative tests, as well as recently developed diagnostic methods. It also provides background information on many pathogens, which are either endemic, non-endemic, or emerging and with different lifecycles that cause diseases of significant importance in a wide variety of hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, background information on pathogens and the disease caused, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Plant Pathology: Techniques and Protocols, Second Edition is the perfect book for plant pathologists and molecular biologists who will use this information to test out the latest research in their laboratories"--Back cover.
Series:
Methods in molecular biology, 1064-3745 ; 1302 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.