Veterinary infection biology : molecular diagnostics and high-throughput strategies / edited by Mónica V. Cunha, Instituto Nacional de Investigação Agrária e Veterinária, Lisbon, Portugal; Centro o de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisbon, Lisbon, Portugal ; João Inácio, Instituto Nacional de Investigação Agrária e Veterinária, Lisbon, Portugal, School of Pharmacy and Biomolecular Sciences, University of Brighton, United Kingdom.
Publisher:
Humana Press,
Copyright Date:
2014
Description:
xix, 536 pages : illustrations (some color) ; 27 cm.
Nested and multiplex real-time PCR using dual-labeled probes : detecting and discriminating Mycobacterium tuberculosis complex members in cultures and animal tissues -- Significance and integration of molecular diagnostics in the framework of veterinary practice -- Biosafety principles and practices for the veterinary diagnostic laboratory -- Veterinary biobank facility : development and management for diagnostic and research purposes -- Biological specimen collection and processing for molecular analysis -- Validation of molecular diagnostic assays and quality assurance and control in the veterinary laboratory -- Molecular approaches to recognize relevant and emerging infectious diseases in animals -- Real-time reverse transcriptase PCR for the detection of Bluetongue virus -- Nested and multiplex real-time PCR using dual-labeled probes : detecting and discriminating Mycobacterium tuberculosis complex members in cultures and animal tissues -- Identification of animal Pasteurellaceae by MALDI-TOF mass spectrometry -- Improved detection of Mycobacterium bovis in bovine tissues using immunomagnetic separation approaches -- Detection of fish pathogens by loop-mediated isothermal amplification (LAMP) technique -- Direct detection of Theileria annulata in bovine blood samples using standard and isothermal DNA amplification approaches -- Reverse line blot hybridization with species-specific oligonucleotide probes : application to piroplasm detection -- DNA microarray-based detection of multiple pathogens : Mycoplasma spp. and Chlamydia spp. -- In situ hybridization with labeled probes : assessment of African swine fever virus in formalin-fixed paraffin-embedded tissues -- Fluorescence in situ hybridization for the tissue detection of bacterial pathogens associated with porcine infections -- Identification of animal Pasteurellaceae by MALDI-TOF mass spectrometry -- Single-nucleotide polymorphism discrimination using high-resolution melting analysis for the genotyping of Bacillus anthracis -- Nucleic-acid testing, new platforms and nanotechnology for point-of-decision diagnosis of animal pathogens -- Molecular typing tools : from pattern recognition to genome-based algorithms -- Characterization of Campylobacter jejuni and Campylobacter coli genotypes in poultry flocks by restriction fragment length polymorphism (RFLP) analysis -- Pulsed-field gel electrophoresis (PFGE) : application in population structure studies of bovine mastitis-causing streptococci -- Multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) using multiplex PCR and multicolor capillary electrophoresis : application to the genotyping of Brucella species -- Multilocus sequence typing (MLST) : markers for the traceability of pathogenic Leptospira strains -- Single-nucleotide polymorphism discrimination using high-resolution melting analysis for the genotyping of Bacillus anthracis -- Proteomics characterization of tick-host-pathogen interactions. Rapid microarray-based genotyping of Chlamydia spp. strains from clinical tissue samples -- Multiplexed genotyping of Bacillus anthracis by Luminex xMap suspension array -- Next-generation sequencing in veterinary medicine : how can the massive amount of information arising from high-throughput technologies improve diagnosis, control, and management of infectious diseases? -- Impact of next-generation technologies on exploring socioeconomically important parasites and developing new interventions -- Functional genomics of tick vectors challenged with the cattle parasite Babesia bigemina -- Metagenomic approaches to disclose disease-associated pathogens : detection of viral pathogens in honeybees -- Proteomics characterization of tick-host-pathogen interactions.
Series:
Methods in molecular biology, 1064-3745 ; 1247 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.