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Author:

Freshney, R. Ian.

Title:

Culture of animal cells : a manual of basic technique / R. Ian Freshney.

Edition:

5th ed.

Publisher:

Wiley-Liss,

Copyright Date:

c2005

Description:

642 p. : ill. (some col.) ; 29 cm.

Subject:

Tissue culture--Laboratory manuals.
Cell culture--Laboratory manuals.
Cells, Cultured.
Culture Techniques.
Tissus (Histologie)--Culture--Manuels de laboratoire.
Cellules--Culture--Manuels de laboratoire.

Notes:

Includes bibliographical references (p. 581-626) and index.

Contents:

Introduction -- Training programs -- Biology of cultured cells -- Laboratory design and layout -- Equipment -- Aseptic technique -- Safety, bioethics, and validation -- Culture vessels and substrates -- Defined media and supplements -- Serum-free media -- Preparation and sterilization -- Primary culture -- Subculture and cell lines -- Cloning and selection -- Cell separation -- Characterization -- Differentiation -- Transformation and immortalization -- Contamination -- Cryopreservation -- Quantitation -- Cytotoxicity -- Culture of specific cell types -- Culture of tumor cells -- Organotypic culture -- Scale-up -- Specialized techniques -- Problem solving -- In conclusion.

ISBN:

9780471453291
0471453293

OCLC:

(OCoLC)58830118

LCCN:

2005281585

Locations:

USUX851 -- Iowa State University - Parks Library (Ames)

PTAX572 -- Stewart Memorial Library (Cedar Rapids)

UWAX953 -- Waldorf University - Luise V. Hanson Library (Forest City)

OVUX522 -- University of Iowa Libraries (Iowa City)