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06291aam a2200433 i 4500 001 134A246C81F911E58D57CB8FDAD10320 003 SILO 005 20151103010200 008 150325t20152015nyua bf 001 0 eng d 010 $a 2015939912 020 $a 1493927140 020 $a 9781493927142 035 $a (OCoLC)907193490 040 $a BTCTA $b eng $e rda $c BTCTA $d YDXCP $d IDL $d OCLCO $d COD $d OCLCO $d CHVBK $d OCLCO $d ZWZ $d OCLCO $d OCLCF $d OCLCO $d NUI $d SILO 245 00 $a Pyrosequencing : $b methods and protocols / $c edited by Ulrich Lehmann, JoÌrg Tost. 250 $a Second edition. 264 1 $a New York : $b Humana Press, $c [2015] 300 $a xiv, 414 pages : $b illustrations (some color) ; $c 27 cm. 490 1 $a Methods molecular biology, $x 1064-3745 ; $v 1315 490 1 $a Springer protocols, $x 1949-2448 500 $a Previous 2007 edition edited by Sharon Marsh. 504 $a Includes bibliographical references and index. 505 00 $g Part II. Analysis of Genetic Variation. $t Analysis of Copy Number Variation by Pyrosequencing® Using Paralogous Sequences / $r Kringen, Marianne Kristiansen -- $t PyroMark® Instruments, Chemistry and Software / $r Kreutz, Martin (and others) -- $t Software-Based Pyrogram® Evaluation / $r Chen, Guoli (and others) -- $t Quantitative Validation and Quality Control of Pyrosequencing® Assays / $r Lehmann, Ulrich -- $g Part II. Analysis of Genetic Variation. $t Extended KRAS and NRAS Mutation Profiling by Pyrosequencing® Assays / $r Jung, Andreas -- $t Universal BRAF State Detection by the Pyrosequencing®-Based U-BRAFv600 Assay / $r Skorokhod, Alexander -- $t Pyrosequencing®-Based Identification of Low-Frequency Mutations Enriched through Enhance ice-COLD-PCR / $r How-Kit, Alexandre and JoÌrg Tost -- $t Analysis of Mutational Hotspots in Routinely Processed Bone Marrow Trephines by Pyrosequencing® / $r Bartels, Stephan and Ulrich Lehmann -- $t Analysis of Copy Number Variation by Pyrosequencing® Using Paralogous Sequences / $r Kringen, Marianne Kristiansen -- 505 80 $t Global Analysis of DNA 5-Methylcytosine Using the Luminometric Methylation Assay, LUMA / $r Luttropp, Karin (and others) -- $t HLA-B and HLA-C Supratyping by Pyrosequencing® / $r Vanni, Irene (and others) -- $t Allele Quantification Pyrosequencing® at Designated SNP Sites to Detect Allelic Expression Imbalance and Loss-of-Heterozygosity / $r Kwok, Chau-To and Megan P. Hitchins -- $g Part III. Analysis of DNA Methylation Patterns. $t Quantitative DNA Methylation Analysis by Pyrosequencing® / $r Roessler, Jessica and Ulrich Lehmann -- $t Quantitative Methylation Analysis of the PCDHB Gene Cluster / $r Banelli, Barbara and Massimo Romani -- $t Assessment of Changes in Global DNA Methylation Levels by Pyrosequencing® of Repetitive Elements / $r Tabish, Ali M. (and others) -- $t Global Analysis of DNA 5-Methylcytosine Using the Luminometric Methylation Assay, LUMA / $r Luttropp, Karin (and others) -- 505 80 $t Detection of Drug-Resistant Mycobacterium tuberculosis / $r Dabrowski, Piotr Wojciech (and others) -- $t Detection of Loss of Imprinting by Pyrosequencing® / $r Tabano, Silvia (and others) -- $t Analysis of DNA Methylation Patterns in Single Blastocysts by Pyrosequencing® / $r Huntriss, John (and others) -- $t Allele-Specific DNA Methylation Detection by Pyrosequencing® / $r Kristensen, Lasse Sommer (and others) -- $t SNP-Based Quantification of Allele-Specific DNA Methylation Patterns by Pyrosequencing® / $r Busato, Florence and JoÌrg Tost -- $t DNA Methylation Analysis of ChIP Products at Single Nucleotide Resolution by Pyrosequencing® / $r Moison, CeÌline (and others) -- $g Part IV. Bacterial Typing and Identification. $t Multiplex Pyrosequencing®: Simultaneous Genotyping Based on SNPs from Distant Genomic Regions / $r Dabrowski, Piotr Wojciech (and others) -- $t Detection of Drug-Resistant Mycobacterium tuberculosis / 505 80 $r Antunes, Joana (and others). $t Tissue-Specific DNA Methylation Patterns in Forensic Samples Detected by Pyrosequencing® / $r Amoako, Kingsley Kwaku -- $t Forensic Analysis of Mitochondrial and Autosomal Markers Using Pyrosequencing® / $r BusÌ, Magdalena M. (and others) -- $t Tissue-Specific DNA Methylation Patterns in Forensic Samples Detected by Pyrosequencing® / $r Antunes, Joana (and others). 520 $a "The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the volume continues with quantitative analysis of genetic variation, ratio of expressed alleles at the RNA level, analysis of DNA methylation, global DNA methylation assays, specialized applications for DNA methylation analysis including loss of imprinting, single blastocyst analysis, allele-specific DNA methylation patterns, DNA methylation patterns associated with specific histone modifications. The volume further details tools and protocols for the detection of viruses and bacteria, and genetic and epigenetic analyses for forensics using Pyrosequencing. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and adaptable, Pyrosequencing: Methods and Protocols, Second Edition will greatly aid doctorial students, postdoctoral investigators and research scientists studying different aspects of genetics and cellular and molecular biology"--Back cover. 650 0 $a DNA $x Analysis $v Laboratory manuals. 650 0 $a Nucleotide sequence $v Laboratory manuals. 650 0 $a Genetics $x Technique $v Laboratory manuals. 650 2 $a Sequence Analysis, DNA $x methods $v Laboratory Manuals. $2 [MeSH] 650 2 $a High-Throughput Nucleotide Sequencing $x methods $v Laboratory Manuals. $2 [MeSH] 700 1 $a Lehmann, Ulrich $c (Molecular biologist), $e editor. 700 1 $a Tost, JoÌrg, $e editor. 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1315, $x 1064-3745 830 0 $a Springer protocols (Series), $x 1949-2448 941 $a 1 952 $l OVUX522 $d 20180117065602.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=134A246C81F911E58D57CB8FDAD10320Initiate Another SILO Locator Search