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06160aam a2200361 i 4500 001 8F6D5CE2E96D11E8978F920F97128E48 003 SILO 005 20181116010210 008 161121t20172017nyua b 001 0deng d 010 $a 2017937361 020 $a 9781493968640 020 $a 1493968645 035 $a (OCoLC)962873593 040 $a YDX $b eng $e rda $c YDX $d IDL $d OCLCO $d NUI $d UtOrBLW $d SILO 245 00 $a MicroRNA detection and target identification : $b methods and protocols / $c edited by Tamas Dalmay. 264 1 $a New York : $b Humana Press, $c [2017] 300 $a xii, 304 pages : $b illustrations (some color) ; $c 26 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 1580 490 1 $a Springer protocols, $x 1949-2448 500 $a "... follow-up of a previous book ... (MicroRNAs in development; published in 2011)"--Preface. 504 $a Includes bibliographical references and index. 505 00 $t Erratum to : Cell-free urinary MicroRNAs expression in small-scale experiments / $r Ludek Zavesky, Eva Jandakova, Radovan Turyna, Daniela Duskova, Lucie Langmeierova, Vit Weinberger, Lubos Minar, Ales Horinek, and Milada Kohoutova. $t 2 High-throughput RT-qPCR for the analysis of circulating MicroRNAs / $r Geok Wee Tan and Lu Ping Tan -- $t Genome-wide comparison of next-generation sequencing and qPCR platforms for microRNA profiling in serum / $r Thorarinn Blondal, Maurizia Rossana Brunetto, Daniela Cavallone, Martin Mikkelsen, Michael Thorsen, Yuan Mang, Hazel Pinheiro, Ferruccio Bonino, and Peter Mouritzen -- $t Small RNA profiling by next-generation sequencing using high-definition adapters / $r Martina Billmeier and Ping Xu -- $t Surface acoustic wave lysis and ion-exchange membrane quantification of exosomal MicroRNA / $r Katherine E. Richards, David B. Go, and Reginald Hill -- $t Droplet microfluidic device fabrication and use for isothermal amplification and detection of MicroRNA / $r Maria Chiara Giuffrida, Roberta D'Agata, and Giuseppe Spoto -- $t Interrogation of functional miRNA-target interactions by CRISPR/Cas9 genome engineering / $r Yale S. Michaels, Qianxin Wu, and Tudor A. Fulga -- $t Cell-free urinary MicroRNAs expression in small-scale experiments / $r Ludek Zavesky, Eva Jandakova, Radovan Turyna, Daniela Duskova, Lucie Langmeierova, Vit Weinberger, Lubos Minar, Ales Horinek, and Milada Kohoutova -- $t Peptide-based isolation of argonaute protein complexes using Ago-APP/ $r Judith Hauptmann and Gunter Meister -- $t Predicting functional MicroRNA-mRNA interactions / $r Zixing Wang and Yin Liu -- $t Computational and experimental identification of tissue-specific microRNA targets / $r Raheleh Amirkhah, Hojjat Naderi Meshkin, Ali Farazmand, John E.J. Rasko, and Ulf Schmitz -- $t sRNAtoolboxVM : small RNA analysis in a virtual machine / $r Cristina GoÌmez-MartiÌn, Ricardo LebroÌn, Antonio Rueda, JoseÌ L. Oliver, and Michael Hackenberg -- $t Assessment of the next generation of animal miRNA target prediction algorithms / $r Thomas Bradley and Simon Moxon -- $t UEA small RNA workbench : a suite of computational tools for small RNA analysis / $r Irina Mohorianu, Matthew Benedict Stocks, Christopher Steven Applegate, Leighton Folkes, and Vincent Moulton -- $t Prediction of miRNA-mRNA interactions using miRGate / $r Eduardo AndreÌs-LeoÌn, Gonzalo GoÌmez-LoÌpez, and David G. Pisano -- $t Detection of microRNAs using chip-based QuantStudio 3D digital PCR / $r Cristina Borzi, Linda Calzolari, Davide Conte, Gabriella Sozzi, and Orazio Fortunato -- $t MiRNA quantitation with microelectrode sensors enabled by enzymeless electrochemical signal amplification / $r Tanyu Wang, Gangli Wang, Didier Merlin, and Emilie Viennois -- $t Robust protocol to quantify circulating cancer biomarker microRNAs / $r Emma Bell, Hannah L. Watson, Shivani Bailey, Matthew J. Murray, and Nicholas Coleman -- $t MicroRNAs, regulatory networks, and comorbidities : decoding complex systems / $r Francesco Russo, Kirstine Belling, Anders Boeck Jensen, Flavia Scoyni, Søren Brunak, and Marco Pellegrini -- $t Label-free direct detection of MiRNAs with poly-silicon nanowire biosensors / $r Jing He, Jianjun Zhu, Bin Jiang, and Yulan Zhao -- $t Erratum to : Cell-free urinary MicroRNAs expression in small-scale experiments / $r Ludek Zavesky, Eva Jandakova, Radovan Turyna, Daniela Duskova, Lucie Langmeierova, Vit Weinberger, Lubos Minar, Ales Horinek, and Milada Kohoutova. 520 $a This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization. The chapters in MicroRNA Detection and Target Identification: Methods and Protocols guide readers through novel approaches such as nanotechnology, microfluidics, based detection methods, analysis of serum and urinary, miRNAs as biomarkers, target identification and experimental approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, MicroRNA Detection and Target Identification: Methods and Protocols aims to ensure successful results in the further study of this vital field. 650 0 $a MicroRNA $v Laboratory manuals. 655 7 $a Laboratory manuals. $2 lcgft $0 http://id.loc.gov/authorities/genreForms/gf2014026120 700 1 $a Dalmay, Tamas, $e editor. $0 http://id.loc.gov/authorities/names/no2011074337 773 18 $w 990001282000202771 $g no:31858067256242 787 0 $a Çt MicroRNAs in development. Çd New York : Springer, c2011 Çw (OCoLC)711076365 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1580. $x 1064-3745 $0 http://id.loc.gov/authorities/names/n92002874 830 0 $a Springer protocols (Series). $x 1949-2448 $0 http://id.loc.gov/authorities/names/no2008180772 941 $a 1 952 $l OVUX522 $d 20181116012137.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=8F6D5CE2E96D11E8978F920F97128E48Initiate Another SILO Locator Search