Directed myogenic differentiation of human induced pluripotent stem cells / Emi Shoji, Knut Woltjen, and Hidetoshi Sakurai -- Doxycycline-inducible system for genetic correction of iPSC disease models / Xiuli Sim [and others] -- Generation and characterization of patient-specific induced pluripotent stem cell for disease modeling / Renuka Sivapatham and Xianmin Zeng -- Modeling genomic imprinting disorders using induced pluripotent stem cells / Stormy J. Chamberlain [and others] -- Generation and characterization of induced pluripotent stem cells from patients with mtDNA mutations / Riikka H. H€am€al€ainen and Anu Suomalainen -- Skin biopsy and patient-specific stem cell lines / Yao Li, Huy V. Nguyen, and Stephen H. Tsang -- Directed myogenic differentiation of human induced pluripotent stem cells / Emi Shoji, Knut Woltjen, and Hidetoshi Sakurai -- Transgene-free disease-specific iPSC generation from fibroblasts and peripheral blood mononuclear cells / Yee Ki Lee [and others] -- Modeling cardiovascular diseases with patient-specific human pluripotent stem cell-derived cardiomyocytes / Paul W. Burridge [and others] -- Calcium imaging in pluripotent stem cell-derived cardiac myocytes / Anna Walter [and others] -- Patient-specific induced pluripotent stem cell models : generation and characterization of cardiac cells / Fabian Zanella and Farah Sheikh -- Differentiation of human pluripotent stem cells to cardiomyocytes under defined conditions / Cathelijne W. van den Berg [and others] -- Generation of cardiomyocytes from pluripotent stem cells / Hiroko Nakahama and Elisa Di Pasquale -- Generation and characterization of patient-specific iPSC model for cardiovascular disease / Yee Ki Lee [and others] -- Transgene-free disease-specific iPSC generation from fibroblasts and peripheral blood mononuclear cells / Xuan Zhang [and others] -- Mitochondrial disease-specific induced pluripotent stem cell models : generation and characterization / Michaela Trilck, Rayk Hubner, and Moritz J. Frech -- Multisystemic disease modeling of liver-derived protein folding disorders using induced pluripotent stem cells (iPSCs) / Amy Leung and George J. Murphy -- In vitro modeling of alcohol-induced liver injury using human-induced pluripotent stem cells / Lipeng Tian, Neha Prasad, and Yoon-Young Jang -- Generation of human induced pluripotent stem cells using RNA-based sendai virus system and pluripotency validation of the resulting cell population / Valeria Chichagova [and others] -- Modeling axonal phenotypes with human pluripotent stem cells / Kyle R. Denton, Chong-Chong Xu, and Xue-Jun Li -- Mitochondrial disease-specific induced pluripotent stem cell models : generation and characterization / Xuan Zhang [and others] -- Generation of integration-free patient specific iPS cells using episomal plasmids under feeder free conditions / Sara Caxaria [and others]. Generation of integration-free patient specific iPS cells using episomal plasmids under feeder free conditions / Sara Caxaria [and others].
Summary:
This volume captures the rapid developments in the field of induced pluripotent stem (iPS) cells, which have provided novel opportunities and approaches both for better understanding a number of human diseases and for developing new platforms for drug development and screening for such diseases. Specifically, representative protocols on various disease models have been collected from labs around the world. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization is an ideal reference for scientists working on furthering iPS research.
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.