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Title:
Embryonic stem cell protocols / edited by Kursad Turksen.
Edition:
Third edition.
Publisher:
Humana Press,
Copyright Date:
2016
Description:
xv, 449 pages : illustrations (some color) ; 27 cm.
Subject:
Stem cells--Laboratory manuals.
Embryos--Laboratory manuals.
Other Authors:
Turksen, Kursad, editor. http://id.loc.gov/authorities/names/n2001009018
Notes:
Includes bibliographical references and index.
Contents:
Imaging pluripotency : time-lapse Analysis of mouse embryonic stem cells / Anna Pezzarossa [and others] -- Simple and efficient method of slow freezing for human embryonic stem cells and induced pluripotent stem cells / Keitaro Imaizumi [and others]. -- Resolving heterogeneity : fluorescence-activated cell sorting of dynamic cell populations from feeder-free mouse embryonic stem cell culture / Jurriaan Holzenspies, Gelo Dela Cruz, and Joshua M. Brickman -- Acquiring ground state pluripotency : switching mouse embryonic stem cells from serum/LlF medium to 2i/LlF medium / Matteo Tosolini and Alice Jouneau -- Generation of embryonic stem cells in rabbits / Marielle Afanassieff, Pierre Osteil, and Pierre Savatier -- Gene transfer into pluripotent stem cells via lentiviral transduction / Ortwin Naujok, Ulf Diekmann, and Matthias Elsner -- Imaging pluripotency : time-lapse Analysis of mouse embryonic stem cells / Anna Pezzarossa [and others] --
Definitive endoderm differentiation of human embryonic stem cells combined with selective elimination of undifferentiated cells by methionine deprivation / Tomonori Tsuyama, Nobuaki Shiraki, and Shoen Kume -- Selection of antibodies interfering with cell surface receptor signaling using embryonic stem cell differentiation / Anna N. Melidoni, Michael R. Dyson, and John McCafferty -- Delivering antisense morpholino oligonucleotides to target telomerase splice variants in human embryonic stem cells / Lida Radan [and others] -- Analysis of mRNA translation rate in mouse embryonic stem cells / Anisa B. Rahim and Leah A. Vardy -- Generation and purification of definitive endoderm cells generated from pluripotent stem cells / Ulf Diekmann and Ortwin Naujok -- Definitive endoderm differentiation of human embryonic stem cells combined with selective elimination of undifferentiated cells by methionine deprivation / Tomonori Tsuyama, Nobuaki Shiraki, and Shoen Kume --
Intracellular calcium measurements for functional characterization of neuronal Phenotypes / Talita Glaser [and others] -- Methods for derivation of multipotent neural crest cells derived from human pluripotent stem cells / John Avery and Stephen Dalton -- From naive to primed pluripotency : in vitro conversion of mouse embryonic stem cells in Epiblast Stem Cells / Matteo Tosolini and Alice Jouneau -- In vitro differentiation of embryonic stem cells into hematopoietic lineage : towards erythroid progenitor's production / Iliana Fauzi, Nicki Panoskaltsis, and Athanasios Mantalaris -- Dopaminergic differentiation of human embryonic stem cells on PA6-derived adipocytes / M. Oktar Guloglu and Anna Larsen -- Intracellular calcium measurements for functional characterization of neuronal Phenotypes / Talita Glaser [and others] --
Concise protocol for siRNA-mediated gene suppression in human embryonic stem cells / Peter F. Renz and Tobias A. Beyer -- Generation of corneal keratocytes from human embryonic stem cells / Andrew J. Hertsenberg and James L. Funderburgh -- Maintenance, transgene delivery, and pluripotency measurement of mouse embryonic stem cells / Tetsuya S. Tanaka -- Generation of a knockout mouse embryonic stem cell line using a paired CRISPR/Cas9 genome engineering tool / Rahel Wettstein, Maxime Bodak, and Constance Ciaudo -- Derivation of neural precursor cells from human embryonic stem cells for DNA methylomic analysis / Ivan Roubal, Sun Joo Park, and Yong Kim -- 26S and PA28-20S proteasome activity in cytosolic extracts from embryonic stem cells / Malin Hernebring -- Concise protocol for siRNA-mediated gene suppression in human embryonic stem cells / Peter F. Renz and Tobias A. Beyer --
Limbal stromal tissue specific stem cells and their differentiation potential to corneal epithelial cells / Kishore Reddy Katikireddy and Ula V. Jurkunas. Generating inner ear organoids from mouse embryonic stem cells / Emma Longworth-Mills, Karl R. Koehler, and Eri Hashino -- Differentiation of adipocytes in monolayer from mouse embryonic stem cells / Ixchelt Cuaranta-Monroy, Zoltan Simandi, and Laszlo Nagy -- Pancreatic differentiation from murine embryonic stem cells / Daisuke Sakano, Nobuaki Shiraki, and Shoen Kume -- Osteogenic differentiation from embryonic stem cells / Yanhong Yu, Carlos Pilquil, and Michal Opas -- Limbal stromal tissue specific stem cells and their differentiation potential to corneal epithelial cells / Kishore Reddy Katikireddy and Ula V. Jurkunas.
Series:
Methods in molecular biology, 1064-3745 ; volume 1341
Springer protocols, 1949-2448
ISBN:
9781493929542
1493929542
9781493929535
1493929534
OCLC:
(OCoLC)934606134
LCCN:
2015951971
Locations:
OVUX522 -- University of Iowa Libraries (Iowa City)

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