Insect virus discovery by metagenomic and cell culture-based approaches / Finny S. Varghese and Ronald P. van Rij. Small RNA isolation from tissues of grapevine and woody plants / Annalisa Giampetruzzi, Michela Chiumenti, Angelantonio Minafra, and Pasquale Saldarelli -- Double-stranded RNA-enriched preparations to identify viroids by next-generation sequencing / Beatriz Navarro and Francesco Di Serio -- Viral double-stranded RNAs (dsRNAs) from plants : alternative nucleic acid substrates for high-throughput sequencing / Armelle Marais, Chantal Faure, Bernard Bergey, and Thierry Candresse -- Workup of human blood samples for deep sequencing of HIV-1 genomes / Marion Cornelissen, Astrid Gall, Antoinette van der Kuyl, Chris Wymant, François Blanquart, Christophe Fraser, and Ben Berkhout -- Monolith chromatography as sample preparation step in virome studies of water samples / Ion Gutiérrez-Aguirre, Denis Kutnjak, Nejc Rački, Matevž Rupar, and Maja Ravnikar -- Viral metagenomics approaches for high-resolution screening of multiplexed arthropod and plant viral communities / Sarah François, Denis Filloux, Emmanuel Fernandez, Mylène Ogliastro, and Philippe Roumagnac -- Different approaches to discover mycovirus associated to marine organisms / Luca Nerva, Giovanna C. Varese, and Massimo Turina -- Use of siRNAs for diagnosis of viruses associated to woody plants in nurseries and stock collections / Nikoletta Czotter, János Molnár, Réka Pesti, Emese Demián, Dániel Baráth, Tünde Varga, and Éva Várallyay -- Use of high-throughput sequencing for the study and diagnosis of plant viruses and viroids in pollen / Kris De Jonghe, Annelies Haegeman, Yoika Foucart, and Martine Maes -- High-resolution screening of viral communities and identification of new pathogens in fish using next-generation sequencing / Arnfinn Lodden Økland, Are Nylund, Ali May, Adalberto Costessi, and Walter Pirovano -- Metagenomic analyses of the viruses detected in mycorrhizal fungi and their host orchid / Hanako Shimura, Chikara Masuta, and Yasunori Koda -- DNA multiple sequence alignment guided by protein domains : the MSA-PAD 2.0 method / Bachir Balech, Alfonso Monaco, Michele Perniola, Monica Santamaria, Giacinto Donvito, Saverio Vicario, Giorgio Maggi, and Graziano Pesole -- From whole-genome shotgun sequencing to viral community profiling : the ViromeScan tool / Simone Rampelli and Silvia Turroni -- Shannon entropy to evaluate substitution rate variation among viral nucleotide positions in datasets of viral siRNAs / Aysan Ghasemzadeh, Marta Małgorzatater Haar, Masoud Shams-bakhsh, Walter Pirovano, and Vitantonio Pantaleo -- Insect virus discovery by metagenomic and cell culture-based approaches / Finny S. Varghese and Ronald P. van Rij.
Summary:
This volume explores the use of viral metagenomics to diagnose known viruses for plant and food production, human and animal health, and identifying viral vectors like insects. The chapters in this book cover topics, such as sRNAs isolation from tissues of grapevines and woody plants, high-resolution screening of arthropod and plant viral communities, identifying new pathogens in fish, detecting viruses in mycorrhizal fungi and their orchid host, and insect virus discovery through metagenomic and cell culture-based approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Viral Metagenomics: Methods and Protocols is a valuable resource for researchers and specialists who are interested in learning more about this evolving field.
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.