Isolation and differentiation of murine primary brown/beige preadipocytes / Hui Yu, Margo Emont, Heejin Jun, and Jun Wu. Method for isolation of stromal vascular fraction cells in a clinically relevant time frame / Joel A. Aronowitz, Ryan A. Lockhart, and Cloe S. Hakakian -- Scaffold-free, size-controlled three-dimensional culture of rabbit adipose-derived stem cells / Christina L. Rettinger, Kai P. Leung, and Rodney K. Chan -- Differentiation of brown adipocyte progenitors derived from human induced pluripotent stem cells / Anne-Laure Hafner, Tala Mohsen-Kanson, and Christian Dani -- Methylcellulose based thermally reversible hydrogels / Anoosha Forghani and Ram Devireddy -- Decellularized adipose tissue scaffolds for soft tissue regeneration and adipose-derived stem/stromal cell delivery / Pascal Morissette Martin, Arthi Shridhar, Claire Yu, Cody Brown, and Lauren E. Flynn -- Induction of skin allograft transplantation tolerance in mice using human adipose derived stromal cells / Anthony D. Foster, Nicholas Clark, and Thomas A. Davis -- High definition confocal imaging modalities for the characterization of tissue-engineered substitutes / Dominique Mayrand and Julie Fradette -- Adipose-derived stromal vascular fraction cells and platelet-rich plasma : basic and clinical implications for tissue engineering therapies in regenerative surgery / Pietro Gentile and Valerio Cervelli -- Myogenic differentiation of ASCs using biochemical and biophysical induction / Pinar Yilgor Huri, Justin Morrissette-McAlmon, and Warren L. Grayson -- Isolation of murine adipose-derived stromal/stem cells for adipogenic differentiation or flow cytometry-based analysis / Gail Kilroy, Marilyn Dietrich, Xiying Wu, Jeffrey M. Gimble, and Z. Elizabeth Floyd -- Three-dimensional magnetic levitation culture system simulating white adipose tissue / Hubert Tseng, Alexes C. Daquinag, Glauco R. Souza, and Mikhail G. Kolonin -- Isolation of human adipose-derived stem cells from lipoaspirates / Jie Li, J. Lowry Curley, Z. Elizabeth Floyd, Xiying Wu, Yuan Di C. Halvorsen, and Jeffrey M. Gimble -- Isolation of murine adipose-derived stromal/stem cells using an explant culture method / Jie Li, Hui Li, and Weidong Tian -- Feline adult adipose tissue-derived multipotent stromal cell isolation and Differentiation / Carmel Fargason, Nan Zhang, and Mandi J. Lopez -- Canine adult adipose tissue-derived multipotent stromal cell isolation and characterization / Wei Duan and Mandi J. Lopez -- Soft tissue reconstruction / Francesco Egro and Kacey G. Marra -- Mechanical stimulation of adipose-derived stem cells for functional tissue engineering of the musculoskeletal system via cyclic hydrostatic pressure, simulated microgravity, and cyclic tensile strain / Rachel C. Nordberg, Josie C. Bodle, and Elizabeth G. Loboa -- Cryopreservation protocols for human adipose tissue derived adult stem cells / Shahensha Shaik and Ram Devireddy -- Bone regeneration with a combination of adipose-derived stem cells and platelet-rich plasma / Satoshi Tajima, Morikuni Tobita, and Hiroshi Mizuno -- Isolation and differentiation of murine primary brown/beige preadipocytes / Hui Yu, Margo Emont, Heejin Jun, and Jun Wu.
Summary:
This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more about the scientific advances in the field of ASC biology and application.
Series:
Methods in molecular biology, 1064-3745 ; 1773 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.