Analysis of retinal vascular plexuses and interplexus connections / Aaron B. Simmons and Peter G. Fuerst. Measuring retinal function in the mouse / Jan Kremers and Naoyuki Tanimoto -- Modeling retinal diseases using genetic approaches in mice / Akiko Maeda and Tadao Maeda -- Cell culture analysis of the phagocytosis of photoreceptor outer segments by primary mouse RPE cells / Roni A. Hazim and David S. Williams -- Two-photon microscopy (TPM) and fluorescence lifetime imaging microscopy (FLIM) of retinal pigment epithelium (RPE) of mice in vivo / Yoko Miura -- RPE visual cycle and biochemical phenotypes of mutant mouse models / Bhubanananda Sahu and Akiko Maeda -- Use of direct current electroretinography for analysis of retinal pigment epithelium function in mouse models / Minzhong Yu and Neal S. Peachey -- Disruption of rhodopsin dimerization in mouse rod photoreceptors by synthetic peptides targeting dimer interface / Sandeep Kumar, Alyssia Lambert, Jon Rainier, and Yingbin Fu -- Experimental approaches for defining the role of the Ca2+-modulated ROS-GC system in retinal rods of mouse / Clint L. Makino, Teresa Duda, Alexandre Pertzev, and Rameshwar K. Sharma -- Microglia analysis in retinal degeneration mouse models / Katharina Dannhausen, Khalid Rashid, and Thomas Langmann -- Determination of mitochondrial oxygen consumption in the retina ex vivo : applications for retinal disease / Yogita K. Adlakha and Anand Swaroop -- Analysis of feedback signaling from horizontal cells to photoreceptors in mice / Arlene A. Hirano, Xue Liu, Nicholas C. Brecha, and Steven Barnes -- Assessment of the absolute excitatory level of the retina by flicker ERG / Naoyuki Tanimoto and Mathias W. Seeliger -- Ex vivo functional evaluation of synaptic transmission from rods to rod bipolar cells in mice / Johan Pahlberg, Anurima Majumder, and Nikolai O. Artemyev -- Functional and morphological analysis of OFF bipolar cells / Chase B. Hellmer and Tomomi Ichinose -- Immunohistochemical phenotyping of mouse amacrine cell subtypes / Denize Atan -- Phenotyping of gap-junctional coupling in the mouse retina / Arndt Meyer, Shubhash Chandra Yadav, and Karin Dedek -- Ganglion cell assessment in rodents with retinal degeneration / Erica L. Fletcher, Ursula Greferath, Susmita Saha, Emily E. Anderson, and Kirstan A. Vessey -- Morphological identification of melanopsin-expressing retinal ganglion cell subtypes in mice / Seul Ki Lee and Tiffany M. Schmidt -- Functional assessment of melanopsin-driven light responses in the mouse : multielectrode array recordings / Shi-Jun Weng, Jordan M. Renna, Wei-Yi Chen, and Xiong-Li Yang -- In vitro assays for mouse müller cell phenotyping through microRNA profiling in the damaged retina / Luis I. Reyes-Aguirre, Heberto Quintero, Brenda Estrada-Leyva, and Mónica Lamas -- Analysis of retinal vascular plexuses and interplexus connections / Aaron B. Simmons and Peter G. Fuerst.
Summary:
The aim of this volume is to provide a wide range of techniques and aspects of mouse retinal phenotyping and characterization. Chapters guide readers through morphological and functional properties of the mouse retina and techniques for the analysis of outer to mid-retina and mid- to inner retina. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Retinal Phenotyping: Methods and Protocols aims to ensure successful results in the further study of this vital field. .
Series:
Methods in molecular biology, 1064-3745 ; 1753 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.