5 Rapid detection of γ-H2Av foci in ex vivo MMS-treated Drosophila imaginal discs / Varandt Y. Khodaverdian and Mitch McVey. Rapid assessment of genotoxicity by flow cytometric detection of cell sycle alterations / Nevenka Bihari -- Ultrasound imaging of DNA-damage effects in live cultured cells and in brain tissue / Hadi Tadayyon, Mehrdad J. Gangeh, Roxana Vlad, Michael C. Kolios, and Gregory J. Czarnota -- Ultrasound imaging of apoptosis : spectroscopic detection of DNA-damage effects in vivo / Hadi Tadayyon, Mehrdad J. Gangeh, Roxana Vlad, Michael C. Kolios, and Gregory J. Czarnota -- Fluorochrome-labeled inhibitors of caspases : expedient in vitro and in vivo markers of apoptotic cells for rapid cytometric analysis / Zbigniew Darzynkiewicz, Hong Zhao, H. Dorota Halicka, Piotr Pozarowski, and Brian Lee -- Fast-halo assay for the detection of DNA damage / Piero Sestili, Cinzia Calcabrini, Anna Rita Diaz, Carmela Fimognari, and Vilberto Stocchi -- Rapid detection of bacterial susceptibility or resistance to quinolones / Fátima Otero, Rebeca Santiso, María Tamayo, Germán Bou, Jaime Gosálvez, and José Luis Fernández -- Rapid detection of apoptosis in cultured mammalian cells / Igor Kudryavtsev, Maria Serebryakova, Liudmila Solovjeva, Maria Svetlova, and Denis Firsanov -- Quick detection of DNase II-type breaks in formalin-fixed tissue sections / Candace L. Minchew and Vladimir V. Didenko -- Express γ-H2AX immunocytochemical detection of DNA damage / Nate Hopp, Jodi Hagen, Birte Aggeler, and Alexander E. Kalyuzhny -- Rapid detection of γ-H2AX by flow cytometry in cultured mammalian cells / Denis Firsanov, Liudmila Solovjeva, Olga Lublinskaya, Valeriy Zenin, Igor Kudryavtsev, Maria Serebryakova, and Maria Svetlova -- Rapid detection of DNA strand breaks in apoptotic cells by flow- and image-cytometry / Hong Zhao and Zbigniew Darzynkiewicz -- Fast micromethod : determination of DNA integrity in cell suspensions and in solid tissues / Nevenka Bihari -- Quantification of DNA damage and repair in mitochondrial, nuclear, and bacterial genomes by real-time PCR / Nadja Patenge -- Zebra tail amplification : accelerated detection of apoptotic blunt-ended DNA breaks by in situ ligation / Vladimir V. Didenko -- Twelve-gel comet assay format for quick examination of DNA damage and repair / Sergey Shaposhnikov and Andrew Collins -- Immunofluorescence analysis of γ-H2AX foci in mammalian fibroblasts at different phases of the cell cycle / Liudmila Solovjeva, Denis Firsanov, Nadezhda Pleskach, and Maria Svetlova -- RAPD-PCR as a rapid approach for the evaluation of genotoxin-induced damage to bacterial DNA / Rosanna Tofalo and Aldo Corsetti -- Rapid detection of γ-H2Av foci in ex vivo MMS-treated Drosophila imaginal discs / Varandt Y. Khodaverdian and Mitch McVey.
Summary:
This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the "research trenches" they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.
Series:
Methods in molecular biology, 1064-3745 ; 1644 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.