Detection of human dicer and argonaute 2 catalytic activity / Marjorie P. Perron ... [et al.] -- Chromatin immunoprecipitation in fission yeast / Thomas A. Volpe and Jessica DeMaio -- Biochemical analyzes of endogenous argonaute complexes immunopurified with anti-argonaute monoclonal antibodies / Keita Miyoshi ... [et al.] -- Mapping of Ago2-GW182 functional interactions / Bing Yao ... [et al.] -- Continuous density gradients to study argonaute and GW182 complexes associated with the endocytic pathway / Derrick Gibbings -- In vitro RISC cleavage assay / Julia Stoehr and Gunter Meister -- Native gel analysis for RISC assembly / Tomoko Kawamata and Yukihide Tomari -- Purification and assembly of human argonaute, dicer, and TRBP complexes / Nabanita De and Ian J. MacRae -- Detection of human dicer and argonaute 2 catalytic activity / Marjorie P. Perron ... [et al.] -- Argonaute pull-down and RISC analysis using 2'-O -methylated oligonucleotides affinity matrices / Guillaume Jannot, Alejandro Vasquez-Rifo, and Martin J. Simard -- Live Cell imaging of argonaute proteins in mammalian cells / Justin M. Pare, Joaquin Lopez-Orozco, and Tom C. Hobman -- Reporter-based assays for analyzing RNA interference in mammalian cells / Lydia V. McClure, Gil Ju Seo, and Christopher S. Sullivan -- Artificial tethering of argonaute proteins for studying their role in translational repression of target mRNAs / Stephanie Eckhardt ... [et al.] -- Efficient system for Let-7 microRNA and GW182 protein-mediated deadenylation in vitro / Marc R. Fabian, Yuri V. Svitkin, and Nahum Sonenberg -- Cell-free microRNA-mediated translation repression in Caenorhabditis elegans / Edlyn Wu and Thomas F. Duchaine -- Argonaute pull-down and RISC analysis using 2'-O -methylated oligonucleotides affinity matrices / Guillaume Jannot, Alejandro Vasquez-Rifo, and Martin J. Simard -- Whole cell proteome regulation by microRNAs captured in a pulsed SILAC mass spectrometry approach / Olivia A. Ebner and Matthias Selbach. Immunoprecipitation of piRNPs and directional, next generation sequencing of piRNAs / Yohei Kirino ... [et al.] -- Generation of an inducible mouse ES cell lines deficient for argonaute proteins / Hong Su and Xiaozhong Wang -- Whole cell proteome regulation by microRNAs captured in a pulsed SILAC mass spectrometry approach / Olivia A. Ebner and Matthias Selbach.
Series:
Methods in molecular biology, 1064-3745 ; 725 Springer protocols
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