Nano-QSAR model for predicting cell viability of human embryonic kidney cells / Serena Manganelli and Emilio Benfenati. Assaying cellular viability using the neutral red uptake assay / Gamze Ates, Tamara Vanhaecke, Vera Rogiers, and Robim M. Rodrigues -- Assessment of cell viability with single-, dual-, and multi-staining methods using image cytometry / Leo Li-Ying Chan, Kelsey J. McCulley, and Sarah L. Kessel -- High-throughput spheroid screens using volume, resazurin reduction, and acid phosphatase Activity / Delyan P. Ivanov, Anna M. Grabowska, and Martin C. Garnett -- Protocol for in vitro high-throughput chemical susceptibility screening in differentiating NT2 stem cells / Ann-Katrin Menzner and Daniel F. Gilbert -- Ferroptosis and cell death analysis by flow cytometry / Daishi Chen, Ilker Y. Eyupoglu, and Nicolai Savaskan -- Assaying mitochondrial respiration as an indicator of cellular metabolism and fitness / Natalia Smolina, Joseph Bruton, Anna Kostareva, and Thomas Sejersen -- ATP-based luciferase viability assay for animal african trypanosomes using a 96-well plate / Keisuke Suganuma, Nthatisi Innocentia Molefe, and Noboru Inoue -- SYBR® green I-based fluorescence assay to assess cell viability of malaria parasites for routine use in compound sreening / Maria Leidenberger, Cornelia Voigtländer, Nina Simon, and Barbara Kappes -- Screening applications to test cellular fitness in Transwell® models after nanoparticle treatment / Bastian Christ, Christina Fey, Alevtina Cubukova, Heike Walles, Sofia Dembski, and Marco Metzger -- Assays for analyzing the role of transport proteins in the uptake and the vectorial transport of substances affecting cell viability / Emir Taghikhani, Martin F. Fromm, and Jörg König -- Metabolite profiling of mammalian cell culture processes to evaluate cellular viability / Isobelle M. Evie, Alan J. Dickson, and Mark Elvin -- Assaying spontaneous network activity and cellular viability -- Using multi-well microelectrode arrays / Jasmine P. Brown, Brittany S. Lynch, Itaevia M. Curry-Chisolm, Timothy J. Shafer, and Jenna D. Strickland -- Quantitative ratiometric Ca2+ imaging to assess cell viability / Oliver Friedrich and Stewart I. Head -- Functional viability : measurement of synaptic vesicle pool sizes / Jana K. Wrosch and Teja W. Groemer -- Phenotyping cellular viability by functional analysis of ion channels : GlyR-targeted screening in NT2-N cells / Katharina Kuenzel, Sepideh Abolpour Mofrad, and Daniel F. Gilbert -- Systematic cell-based phenotyping of missense alleles / Aenne S. Thormählen and Heiko Runz -- Second harmonic generation microscopy of muscle cell morphology and dynamics / Andreas Buttgereit -- Assessment of population and ECM production using multiphoton microscopy as an indicator of cell viability / Martin Vielreicher and Oliver Friedrich -- Average rheological quantities of cells in monolayers / Haider Dakhil and Andreas Wierschem -- Measurement of cellular behavior by electrochemical impedance sensing / Simin Öz, Achim Breiling, and Christian Maercker -- Nano-QSAR model for predicting cell viability of human embryonic kidney cells / Serena Manganelli and Emilio Benfenati.
Summary:
This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell Viability Assays: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Series:
Methods in Molecular Biology, 1064-3745 ; 1601 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.