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Title:
Plant transposable elements : methods and protocols / edited by Thomas Peterson.
Publisher:
Humana Press,
Copyright Date:
2013
Description:
xv, 324 pages : illustrations (some color) ; 26 cm.
Subject:
Transposons--Laboratory manuals.
Plant genetic transformation.
Genome, Plant--genetics--Laboratory Manuals
DNA Transposable Elements--Laboratory Manuals.
Other Authors:
Peterson, Thomas A., editor.
Springer Science+Business Media, copyright holder.
Notes:
Includes bibliographical references and index.
Contents:
Using MuDR/Mu transposons in directed tagging strategies / V. Walbot and J. Questa -- Distinguishing variable phenotypes from variegation caused by transposon activities / V. Walbot -- Using transposons for genetic mosaic analysis of plant development / P.W. Becraft -- Survey of natural and transgenic gene markers used to monitor transposon activity / L. Krishnaswamy and T. Peterson -- Molecular biology of maize Ac/Ds elements: An overview / K. Lazarow, M.L. Doll and R. Kunze -- Gene tagging with engineered Ds elements in maize / Y. Li, G. Segal, Q. Wangm H.K. Donner -- Plant regeneration methods for rapid generation of a large scale Ds transposant population in rice / Y.H. Xuan [and nine others] -- Isolation of sequences flanking Ac insertion sites by Ac casting / D. Wang and T. Peterson -- Regulation of the mutator system of transposons in maize / D. Lisch -- Using MuDR/Mu transposons in directed tagging strategies / V. Walbot and J. Questa --
Transposon display: A versatile method for transposon tagging / M. Vandenbussche, J. Zethof and T. Gerats -- Digestion-ligation-amplification (DLA): A simple genome walking method to amplify unknown sequences flanking mutator (Mu) transposons and thereby facilitate gene cloning / S. Liu, A.P. Hsia and P.S. Schnable -- Molecular genetics and epigenetics of CACTA elements / N.V. Fedoroff -- Activation tagging using the maize En-I transposon system for the identification of abiotic stress resistance genes in arabidopsis / A. Harb and A. Pereira -- Reverse genetics in rice using Tos17 / D. Mieulet, A. Dievart, G. Droc, N. Lanau, E. Guiderdoni -- Identification and applications of the Petunia Class II Act1/dTph1 transposable element system / T. Gerats, J. Zethof and M. Vandenbussche -- Transposon display: A versatile method for transposon tagging / M. Vandenbussche, J. Zethof and T. Gerats --
Tenest 2.0: Computational annotation and visualization of nested transposable elements / B.A. Kronmiller and R.P. Wise. Use of next generation sequencing (NGS) technologies for the genome-wide detection of transposition / M. Elbaidouri, C. Chaparro and O. Panaud -- Overview of repeat annotation and de novo repeat identification / N. Jiang -- Computational methods for identification of DNA transposons / N. Jiang -- Tenest 2.0: Computational annotation and visualization of nested transposable elements / B.A. Kronmiller and R.P. Wise.
Series:
Methods in molecular biology, 1064-3745 ; 1057
Springer protocols, 1949-2448
ISBN:
1627035672
9781627035675
OCLC:
(OCoLC)841895407
LCCN:
2013942868
Locations:
USUX851 -- Iowa State University - Parks Library (Ames)
OVUX522 -- University of Iowa Libraries (Iowa City)

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